In vivo magnetic resonance imaging of catheter-based vascular gene transfer

X. Yang, E. Atalar, D. Li, J. M. Serfaty, D. Wang, A. Kumar, L. Cheng

Research output: Contribution to journalConference articlepeer-review

Abstract

The purpose of this study was to develop an in vivo imaging tool to monitor vascular gene transfer. We produced gadolinium/blue-dye and gadolinium/gene-vector media by mixing Magnevist with a trypan-blue or a lentiviral vector carrying a green fluorescent protein (GFP) gene. The gadolinium was used as an imaging marker for MRI to visualize vessel wall enhancement, while the blue-dye/GFP was used as a tissue stain marker for histology/immunohistochemistry to confirm the success of the transfer. Using Remedy gene delivery catheters, we transferred the gadolinium/blue-dye (n=8) or gadolinium/GFP-lentivirus (n=4) into the arteries of 12 pigs, monitored under high-resolution MR imaging. This technical development enabled dynamic visualization of: (i) where the gadolinium/genes distributed; (ii) how satisfactorily the target portion was marked; and (iii) whether the gene transfer procedure caused complications. Our study represents the first direct evidence that catheter-based vascular gene delivery/distribution can be monitored by MR imaging in vivo.

Original languageEnglish (US)
Pages (from-to)2272-2275
Number of pages4
JournalAnnual Reports of the Research Reactor Institute, Kyoto University
Volume3
StatePublished - Dec 1 2001
Event23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey
Duration: Oct 25 2001Oct 28 2001

Keywords

  • Cardiovascular disease
  • Magnetic resonance imaging
  • Vascular gene therapy

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Mechanical Engineering

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