Abstract
The purpose of this study was to develop an in vivo imaging tool to monitor vascular gene transfer. We produced gadolinium/blue-dye and gadolinium/gene-vector media by mixing Magnevist with a trypan-blue or a lentiviral vector carrying a green fluorescent protein (GFP) gene. The gadolinium was used as an imaging marker for MRI to visualize vessel wall enhancement, while the blue-dye/GFP was used as a tissue stain marker for histology/immunohistochemistry to confirm the success of the transfer. Using Remedy gene delivery catheters, we transferred the gadolinium/blue-dye (n=8) or gadolinium/GFP-lentivirus (n=4) into the arteries of 12 pigs, monitored under high-resolution MR imaging. This technical development enabled dynamic visualization of: (i) where the gadolinium/genes distributed; (ii) how satisfactorily the target portion was marked; and (iii) whether the gene transfer procedure caused complications. Our study represents the first direct evidence that catheter-based vascular gene delivery/distribution can be monitored by MR imaging in vivo.
Original language | English (US) |
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Pages (from-to) | 2272-2275 |
Number of pages | 4 |
Journal | Annual Reports of the Research Reactor Institute, Kyoto University |
Volume | 3 |
State | Published - 2001 |
Externally published | Yes |
Event | 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey Duration: Oct 25 2001 → Oct 28 2001 |
Keywords
- Cardiovascular disease
- Magnetic resonance imaging
- Vascular gene therapy
ASJC Scopus subject areas
- Energy Engineering and Power Technology
- Mechanical Engineering