In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

Viacheslav Mazlin, Peng Xiao, Eugénie Dalimier, Kate Grieve, Kristina Irsch, José Sahel, Mathias Fink, Claude Boccara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Despite obvious improvements in visualization of the in vivo cornea through the faster imaging speeds and higher axial resolutions, cellular imaging stays unresolvable task for OCT, as en face viewing with a high lateral resolution is required. The latter is possible with FFOCT, a method that relies on a camera, moderate numerical aperture (NA) objectives and an incoherent light source to provide en face images with a micrometer-level resolution. Recently, we for the first time demonstrated the ability of FFOCT to capture images from the in vivo human cornea1. In the current paper we present an extensive study of appearance of healthy in vivo human corneas under FFOCT examination. En face corneal images with a micrometer-level resolution were obtained from the three healthy subjects. For each subject it was possible to acquire images through the entire corneal depth and visualize the epithelium structures, Bowman's layer, sub-basal nerve plexus (SNP) fibers, anterior, middle and posterior stroma, endothelial cells with nuclei. Dimensions and densities of the structures visible with FFOCT, are in agreement with those seen by other cornea imaging methods. Cellular-level details in the images obtained together with the relatively large field-of-view (FOV) and contactless way of imaging make this device a promising candidate for becoming a new tool in ophthalmological diagnostics.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XXVIII
PublisherSPIE
Volume10474
ISBN (Electronic)9781510614338
DOIs
StatePublished - Jan 1 2018
Event28th Conference on Ophthalmic Technologies - San Francisco, United States
Duration: Jan 27 2018Jan 28 2018

Other

Other28th Conference on Ophthalmic Technologies
CountryUnited States
CitySan Francisco
Period1/27/181/28/18

Fingerprint

cornea
Optical tomography
Optical Coherence Tomography
Cornea
tomography
Imaging techniques
micrometers
Cell Nucleus
Nerve Fibers
Endothelial cells
Healthy Volunteers
Epithelium
nerve fibers
Endothelial Cells
epithelium
Light sources
Light
Equipment and Supplies
numerical aperture
guy wires

Keywords

  • Cornea
  • Cornea imaging
  • Full-field optical coherence tomography
  • Human eye imaging
  • Imaging systems
  • In vivo imaging
  • Ophthalmic devices
  • Optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Mazlin, V., Xiao, P., Dalimier, E., Grieve, K., Irsch, K., Sahel, J., ... Boccara, C. (2018). In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography. In Ophthalmic Technologies XXVIII (Vol. 10474). [104740S] SPIE. https://doi.org/10.1117/12.2288947

In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography. / Mazlin, Viacheslav; Xiao, Peng; Dalimier, Eugénie; Grieve, Kate; Irsch, Kristina; Sahel, José; Fink, Mathias; Boccara, Claude.

Ophthalmic Technologies XXVIII. Vol. 10474 SPIE, 2018. 104740S.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mazlin, V, Xiao, P, Dalimier, E, Grieve, K, Irsch, K, Sahel, J, Fink, M & Boccara, C 2018, In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography. in Ophthalmic Technologies XXVIII. vol. 10474, 104740S, SPIE, 28th Conference on Ophthalmic Technologies, San Francisco, United States, 1/27/18. https://doi.org/10.1117/12.2288947
Mazlin V, Xiao P, Dalimier E, Grieve K, Irsch K, Sahel J et al. In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography. In Ophthalmic Technologies XXVIII. Vol. 10474. SPIE. 2018. 104740S https://doi.org/10.1117/12.2288947
Mazlin, Viacheslav ; Xiao, Peng ; Dalimier, Eugénie ; Grieve, Kate ; Irsch, Kristina ; Sahel, José ; Fink, Mathias ; Boccara, Claude. / In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography. Ophthalmic Technologies XXVIII. Vol. 10474 SPIE, 2018.
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