In Vivo Imaging of Composite Hydrogel Scaffold Degradation Using CEST MRI and Two-Color NIR Imaging

Wei Zhu, Chengyan Chu, Shreyas Kuddannaya, Yue Yuan, Piotr Walczak, Anirudha Singh, Xiaolei Song, Jeff W.M. Bulte

Research output: Contribution to journalArticle

Abstract

Hydrogel scaffolding of stem cells is a promising strategy to overcome initial cell loss and manipulate cell function post-transplantation. Matrix degradation is a requirement for downstream cell differentiation and functional tissue integration, which determines therapeutic outcome. Therefore, monitoring of hydrogel degradation is essential for scaffolded cell replacement therapies. It is shown here that chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) can be used as a label-free imaging platform for monitoring the degradation of crosslinked hydrogels containing gelatin (Gel) and hyaluronic acid (HA), of which the stiffness can be fine-tuned by varying the ratio of the Gel:HA. By labeling Gel and HA with two different near-infrared (NIR) dyes having distinct emission frequencies, it is shown here that the HA signal remains stable for 42 days, while the Gel signal gradually decreases to <25% of its initial value at this time point. Both imaging modalities are in excellent agreement for both the time course and relative value of CEST MRI and NIR signals (R2 = 0.94). These findings support the further use of CEST MRI for monitoring biodegradation and optimizing of gelatin-containing hydrogels in a label-free manner.

Original languageEnglish (US)
Article number1903753
JournalAdvanced Functional Materials
DOIs
StatePublished - Jan 1 2019

Fingerprint

Hydrogel
gelatins
Infrared imaging
Magnetic resonance
Gelatin
Hyaluronic acid
Hydrogels
Scaffolds
magnetic resonance
Hyaluronic Acid
degradation
Color
saturation
color
Imaging techniques
Degradation
composite materials
Composite materials
acids
cells

Keywords

  • biodegradation
  • CEST MRI
  • gelatin
  • hyaluronic acid
  • hydrogels
  • NIR imaging
  • scaffolds

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrochemistry

Cite this

In Vivo Imaging of Composite Hydrogel Scaffold Degradation Using CEST MRI and Two-Color NIR Imaging. / Zhu, Wei; Chu, Chengyan; Kuddannaya, Shreyas; Yuan, Yue; Walczak, Piotr; Singh, Anirudha; Song, Xiaolei; Bulte, Jeff W.M.

In: Advanced Functional Materials, 01.01.2019.

Research output: Contribution to journalArticle

@article{b3e56ccbf94a458bbfe1c33920fc1417,
title = "In Vivo Imaging of Composite Hydrogel Scaffold Degradation Using CEST MRI and Two-Color NIR Imaging",
abstract = "Hydrogel scaffolding of stem cells is a promising strategy to overcome initial cell loss and manipulate cell function post-transplantation. Matrix degradation is a requirement for downstream cell differentiation and functional tissue integration, which determines therapeutic outcome. Therefore, monitoring of hydrogel degradation is essential for scaffolded cell replacement therapies. It is shown here that chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) can be used as a label-free imaging platform for monitoring the degradation of crosslinked hydrogels containing gelatin (Gel) and hyaluronic acid (HA), of which the stiffness can be fine-tuned by varying the ratio of the Gel:HA. By labeling Gel and HA with two different near-infrared (NIR) dyes having distinct emission frequencies, it is shown here that the HA signal remains stable for 42 days, while the Gel signal gradually decreases to <25{\%} of its initial value at this time point. Both imaging modalities are in excellent agreement for both the time course and relative value of CEST MRI and NIR signals (R2 = 0.94). These findings support the further use of CEST MRI for monitoring biodegradation and optimizing of gelatin-containing hydrogels in a label-free manner.",
keywords = "biodegradation, CEST MRI, gelatin, hyaluronic acid, hydrogels, NIR imaging, scaffolds",
author = "Wei Zhu and Chengyan Chu and Shreyas Kuddannaya and Yue Yuan and Piotr Walczak and Anirudha Singh and Xiaolei Song and Bulte, {Jeff W.M.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1002/adfm.201903753",
language = "English (US)",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley-VCH Verlag",

}

TY - JOUR

T1 - In Vivo Imaging of Composite Hydrogel Scaffold Degradation Using CEST MRI and Two-Color NIR Imaging

AU - Zhu, Wei

AU - Chu, Chengyan

AU - Kuddannaya, Shreyas

AU - Yuan, Yue

AU - Walczak, Piotr

AU - Singh, Anirudha

AU - Song, Xiaolei

AU - Bulte, Jeff W.M.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Hydrogel scaffolding of stem cells is a promising strategy to overcome initial cell loss and manipulate cell function post-transplantation. Matrix degradation is a requirement for downstream cell differentiation and functional tissue integration, which determines therapeutic outcome. Therefore, monitoring of hydrogel degradation is essential for scaffolded cell replacement therapies. It is shown here that chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) can be used as a label-free imaging platform for monitoring the degradation of crosslinked hydrogels containing gelatin (Gel) and hyaluronic acid (HA), of which the stiffness can be fine-tuned by varying the ratio of the Gel:HA. By labeling Gel and HA with two different near-infrared (NIR) dyes having distinct emission frequencies, it is shown here that the HA signal remains stable for 42 days, while the Gel signal gradually decreases to <25% of its initial value at this time point. Both imaging modalities are in excellent agreement for both the time course and relative value of CEST MRI and NIR signals (R2 = 0.94). These findings support the further use of CEST MRI for monitoring biodegradation and optimizing of gelatin-containing hydrogels in a label-free manner.

AB - Hydrogel scaffolding of stem cells is a promising strategy to overcome initial cell loss and manipulate cell function post-transplantation. Matrix degradation is a requirement for downstream cell differentiation and functional tissue integration, which determines therapeutic outcome. Therefore, monitoring of hydrogel degradation is essential for scaffolded cell replacement therapies. It is shown here that chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) can be used as a label-free imaging platform for monitoring the degradation of crosslinked hydrogels containing gelatin (Gel) and hyaluronic acid (HA), of which the stiffness can be fine-tuned by varying the ratio of the Gel:HA. By labeling Gel and HA with two different near-infrared (NIR) dyes having distinct emission frequencies, it is shown here that the HA signal remains stable for 42 days, while the Gel signal gradually decreases to <25% of its initial value at this time point. Both imaging modalities are in excellent agreement for both the time course and relative value of CEST MRI and NIR signals (R2 = 0.94). These findings support the further use of CEST MRI for monitoring biodegradation and optimizing of gelatin-containing hydrogels in a label-free manner.

KW - biodegradation

KW - CEST MRI

KW - gelatin

KW - hyaluronic acid

KW - hydrogels

KW - NIR imaging

KW - scaffolds

UR - http://www.scopus.com/inward/record.url?scp=85068648634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068648634&partnerID=8YFLogxK

U2 - 10.1002/adfm.201903753

DO - 10.1002/adfm.201903753

M3 - Article

AN - SCOPUS:85068648634

JO - Advanced Functional Materials

JF - Advanced Functional Materials

SN - 1616-301X

M1 - 1903753

ER -