In vivo imaging of baboon and human dopamine transporters by positron emission tomography using [11C]WIN 35,428

Dean F. Wong, Babington Yung, Robert F. Dannals, Elias K. Shaya, Hayden T. Ravert, Catherine A. Chen, Boon Chan, Traci Folio, Ursula Scheffel, George A. Ricaurte, John L. Neumeyer, Henry N. Wagner, Michael J. Kuhar

Research output: Contribution to journalArticle

Abstract

[11C]WIN 35,428 was evaluated as a specific in vivo radioligand for the dopamine transporter site by PET scanning in nonhuman primates and humans. In studies with a baboon (Papio anubis), [11C]WIN 35,428 accumulated in brain regions containing dopamine transporters, i.e., the striata. This accumulation was Partially blocked by prior administration of (−)cocaine (4 mg/kg, i.v. ). Placement of a unilateral lesion of dopamine‐containing nerve terminals with MPTP resulted in a unilateral reduction in [11C]WIN 35,428 accumulation in the striatum on the side of the lesion. Imaging of D2 dopamine receptors with [11C]NMSP in the same MPTP‐treated animals showed much less reduction in the postsynaptic D2 dopamine receptors as compared to the much larger reduction in the dopamine transporters labeled with [11C]WIN 35,428. A total of ten normal human volunteers (five males and five females) with ages ranging from 19 to 81 years were studied. The caudate/cerebellar and putamen/cerebellar ratios ranged from 4.4 to 5.7 90 min after injection of the tracer. Preliminary kinetic modeling with arterial plasma sampling resulted in an average binding potential (K3/K4) of 4.98 in the caudate nucleus and 5.13 in putamen. To demonstrate in vivo blockade with dopamine reuptake inhibitors, two subjects received prior oral doses of 6 mg mazindol. Subject 5 had significant reductions of 29% in the caudate/cerebellar ratio at 90 min, 35% in the putamen/cerebellar ratio at 90 min, 45% in the caudate k3/k4 ratio from 6.7 to 3.7, and 46% in the putamen k3/k4 from 4.7 to 2.5. Subject 8 had significant reductions of 20% in both the caudate/cerebellar ratio and the putamen/cerebellar ratio at 90 min. During the human PET studies, a number of neuropsychological tests and physiological measurements were performed. No significant changes were found after administration of the [11C]WIN 35,428 alone. Taken together, these data indicate that [11C]WIN 35,428 is a promising radioligand for future studies of neuropsychiatric disorders that involve the dopamine transporter site. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)130-142
Number of pages13
JournalSynapse
Volume15
Issue number2
DOIs
StatePublished - Oct 1993

Keywords

  • Cocaine
  • Dopamine reuptake site
  • Dopamine transporter
  • PET

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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    Wong, D. F., Yung, B., Dannals, R. F., Shaya, E. K., Ravert, H. T., Chen, C. A., Chan, B., Folio, T., Scheffel, U., Ricaurte, G. A., Neumeyer, J. L., Wagner, H. N., & Kuhar, M. J. (1993). In vivo imaging of baboon and human dopamine transporters by positron emission tomography using [11C]WIN 35,428. Synapse, 15(2), 130-142. https://doi.org/10.1002/syn.890150205