In vivo facilitation of Tetrahymena group I intron splicing in Escherichia coli pre-ribosomal RNA

Fan Zhang, Edward S. Ramsay, Sarah A. Woodson

Research output: Contribution to journalArticle

Abstract

The observation that the large ribosomal RNA intron of Tetrahymena is spliced 20-50-fold more rapidly in vivo than in vitro (Brehm SL, Cech TR, 1983, Biochemistry 22:2390-2397; Bass BL, Cech TR, 1984, Nature 308:820-826) suggests facilitation of RNA folding in vivo. To determine whether a specific group I splicing factor is required in Tetrahymena, the intron was inserted into the analogous position of the Escherichia coli 23S rRNA. We report that the intron is rapidly excised from pre-rRNA in bacteria and that the magnitude of the in vivo rate enhancement is similar to that in Tetrahymena. These results demonstrate that a species-specific protein is not required. Instead, a common mechanism of assisting RNA folding is sufficient to accelerate the removal of self-splicing introns from ribosomal RNA.

Original languageEnglish (US)
Pages (from-to)284-292
Number of pages9
JournalRNA
Volume1
Issue number3
StatePublished - Dec 1 1995

Keywords

  • 23S rRNA
  • RNA folding
  • Splicing factor

ASJC Scopus subject areas

  • Molecular Biology

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  • Cite this

    Zhang, F., Ramsay, E. S., & Woodson, S. A. (1995). In vivo facilitation of Tetrahymena group I intron splicing in Escherichia coli pre-ribosomal RNA. RNA, 1(3), 284-292.