TY - JOUR
T1 - In vivo expression of mRNAs encoding hypoxia-inducible factor 1
AU - Wiener, Charles M.
AU - Booth, Greg
AU - Semenza, Gregg L.
N1 - Funding Information:
We thank A. Choi for providing the 18S rRNA oligonucleotide. We are grateful to S. Leung and R. Roe for technical assistance. G.L.S. is an Established Investigator of the American Heart Association and this work was supported in part by grants from the American Heart Associate, the Lucille P. Markey Charitable Trust, and the National Institutes of Health (DK39869 and HL55338) to G.L.S.
PY - 1996/8/14
Y1 - 1996/8/14
N2 - Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix transcription factor that regulates genes whose products play key roles in maintaining O2 homeostasis. We have previously demonstrated that HIF-1 mRNA, protein, and DNA-binding activity are induced when mammalian tissue culture cells are subjected to hypoxia. In this paper, we report our analysis of HIF-1 mRNA expression in vivo. We demonstrate expression of HIF-1α and HIF-1β (ARNT) mRNA in all human, rat, and mouse organs assayed and show for the first time that HIF-1 mRNA expression was induced in brain, kidney, and lung when rats or mice were exposed to reduced ambient O2 concentrations for 30 to 60 min. The ubiquitous in vivo expression of HIF-1α and HIF-1β (ARNT) mRNA is consistent with the proposed role of HIF-1 in coordinating adaptive transcriptional responses to hypoxia.
AB - Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix transcription factor that regulates genes whose products play key roles in maintaining O2 homeostasis. We have previously demonstrated that HIF-1 mRNA, protein, and DNA-binding activity are induced when mammalian tissue culture cells are subjected to hypoxia. In this paper, we report our analysis of HIF-1 mRNA expression in vivo. We demonstrate expression of HIF-1α and HIF-1β (ARNT) mRNA in all human, rat, and mouse organs assayed and show for the first time that HIF-1 mRNA expression was induced in brain, kidney, and lung when rats or mice were exposed to reduced ambient O2 concentrations for 30 to 60 min. The ubiquitous in vivo expression of HIF-1α and HIF-1β (ARNT) mRNA is consistent with the proposed role of HIF-1 in coordinating adaptive transcriptional responses to hypoxia.
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U2 - 10.1006/bbrc.1996.1199
DO - 10.1006/bbrc.1996.1199
M3 - Article
C2 - 8753788
AN - SCOPUS:0030583293
SN - 0006-291X
VL - 225
SP - 485
EP - 488
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -