In vivo estimation of cerebral blood flow, oxygen consumption and glucose metabolism in the pig by [15O]water injection, [15O]oxygen inhalation and dual injections of [18F]fluorodeoxyglucose

P. H. Poulsen, D. F. Smith, L. Østergaard, E. H. Danielsen, A. Gee, S. B. Hansen, J. Astrup, A. Gjedde

Research output: Contribution to journalArticle

Abstract

There is a need for suitable non-primate laboratory animals for studies of brain function by positron emission tomography (PET). To provide a comparative index of the circulatory physiology of the pig, we have applied novel PET tracer methodology to seven anaesthetized pigs, and measured cerebral regional oxygen consumption (CMR(O2)), cerebral blood flow (CBF), and cerebral glucose metabolism (CMR(glc)). Blood flow and flow-metabolism couple were estimated for selected cerebral regions of interest. We found an average hemispheric CMR(O2) of 171 ± 18 μmol/100 cm3/min. Individual hemispheric CBF measurements varied between 33 and 41 ml/100 cm3/min, with an average of 37 ± 3 ml/100 cm3/min at an average Pa(CO2) of 4.3 ± 0.9 kPa. The blood flow dependency on arterial P(CO2) was calculated from the results of the carbon dioxide response in two pigs in which the CBF measurements obeyed the equation CBF (ml/100 cm3/min) = 8.9 Pa(CO2) (kPa). In each pig, CMR(glc)was studied twice with a double-injection FDG method. In the first session, the values of CMR(glc) averaged 27 ± 3 and 23 ± 4 μmol/100 cm3/min, estimated by multilinear and linear regression analysis, respectively. In the second session, the corresponding averages were 27 ± 3 and 24 ± 3 μmol/100 cm3/min, respectively. The average oxygen extraction fraction was 0.46 ± 0.09 and the oxygen-glucose ratio was 6.1 ± 0.8. The findings indicate that the pig is suitable for PET studies of cerebral blood flow, cerebral oxygen consumption and glucose metabolism.

Original languageEnglish (US)
Pages (from-to)199-209
Number of pages11
JournalJournal of Neuroscience Methods
Volume77
Issue number2
DOIs
StatePublished - Dec 1 1997

Keywords

  • Carbon dioxide response
  • Cerebral metabolic rates
  • Lumped constant
  • Oxygen extraction fraction
  • Oxygen-glucose metabolic ratio
  • Positron emission tomography

ASJC Scopus subject areas

  • Neuroscience(all)

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