In vivo electroporation of developing mouse retina

Jimmy de Melo, Seth Blackshaw

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In vivo electroporation enables the transformation of retinal tissue with engineered DNA plasmids, facilitating the selective expression of desired gene products. This method achieves plasmid transfer via the application of an external electrical field, which both generates a transient increase in the permeability of cell plasma membranes, and promotes the incorporation of DNA plasmids by electrophoretic transfer through the permeabilized membranes. Here we describe a method for the preparation, injection, and electroporation of DNA plasmids into neonatal mouse retinal tissue. This method can be utilized to perform gain of function or loss of function studies in the mouse. Experimental design is limited only by construct availability.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages101-111
Number of pages11
Volume1715
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1715
ISSN (Print)1064-3745

Keywords

  • Electroporation
  • Gain of function
  • Gene expression
  • In vivo
  • Loss of function
  • Plasmid
  • Retina
  • Subretinal injection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    de Melo, J., & Blackshaw, S. (2018). In vivo electroporation of developing mouse retina. In Methods in Molecular Biology (Vol. 1715, pp. 101-111). (Methods in Molecular Biology; Vol. 1715). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7522-8_8