In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration

Saijilafu, Bo Yin Zhang, Feng Quan Zhou

Research output: Contribution to journalArticle

Abstract

Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

Original languageEnglish (US)
Pages (from-to)167-175
Number of pages9
JournalMethods in Molecular Biology
Volume1162
DOIs
StatePublished - Jan 1 2014

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Keywords

  • Adult mouse sensory neurons
  • Axon regeneration
  • Dorsal root ganglion
  • In vivo electroporation
  • Peripheral nervous system

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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