In vivo efficacy of a novel double liposome as an oral dosage form of salmon calcitonin

Yoshinori Ebato, Yoshinori Kato, Hiraku Onishi, Tsuneji Nagai, Yoshiharu Machida

Research output: Contribution to journalArticlepeer-review

Abstract

A simple method for the preparation of the inner liposomes for double liposomes (DL) was developed. The encapsulation efficiency of erythrosine in liposomes prepared by this new method is superior to that of the previous method because of the concentration of the drug in the lipid membrane. To evaluate the usefulness of DL prepared by the glass-filter method modified in this study as an oral dosage form of salmon calcitonin (SCT), a suspension of liposomes containing SCT was administered to rats at a dose of 10 μg SCT/kg. Each type of DL showed better efficacy than its inner liposomes alone. The decrease in plasma calcium level was dependent on the electrical charge and particle size of the inner liposomes. The hypocalcemic efficacy of DL encapsulating SCT-loading cationic liposomes relative to that after subcutaneous administration of SCT at a dose of 1 μg/kg was 6.47%, which was the largest value obtained. These results indicated that not only the particle size but also the electrical charge of inner liposomes affect intestinal absorption. This study verified that the efficacy was increased because of the decrease in diameter of the inner liposomes and the use of lipid with a positive charge. These findings concluded that DL might be useful as an oral dosage form of SCT.

Original languageEnglish (US)
Pages (from-to)253-257
Number of pages5
JournalDrug Development Research
Volume58
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Keywords

  • Double liposomes
  • Glass-filter method
  • Oral administration
  • Plasma calcium level
  • Salmon calcitonin

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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