In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens

Steffen Jung, Derya Unutmaz, Phillip Wong, Gen Ichiro Sano, Kenia De Los Santos, Tim Sparwasser, Shengji Wu, Sri Vuthoori, Kyung Ko, Fidel Zavala, Eric G. Pamer, Dan R. Littman, Richard A. Lang

Research output: Contribution to journalArticle

Abstract

Cytotoxic T lymphocytes (CTL) respond to antigenic peptides presented on MHC class I molecules. On most cells, these peptides are exclusively of endogenous, cytosolic origin. Bone marrow-derived antigen-presenting cells, however, harbor a unique pathway for MHC I presentation of exogenous antigens. This mechanism permits cross-presentation of pathogen-infected cells and the priming of CTL responses against intracellular microbial infections. Here, we report a novel diphtheria toxin-based system that allows the inducible, short-term ablation of dendritic cells (DC) in vivo. We show that in vivo DC are required to cross-prime CTL precursors. Our results thus define a unique in vivo role of DC, i.e., the sensitization of the immune system for cell-associated antigens. DC-depleted mice fail to mount CTL responses to infection with the intracellular bacterium Listeria monocytogenes and the rodent malaria parasite Plasmodium yoelii.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalImmunity
Volume17
Issue number2
DOIs
StatePublished - Aug 2002
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Jung, S., Unutmaz, D., Wong, P., Sano, G. I., De Los Santos, K., Sparwasser, T., Wu, S., Vuthoori, S., Ko, K., Zavala, F., Pamer, E. G., Littman, D. R., & Lang, R. A. (2002). In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens. Immunity, 17(2), 211-220. https://doi.org/10.1016/S1074-7613(02)00365-5