In vivo characterization of extracellular GABA release in the caudate nucleus and prefrontal cortex of the rhesus monkey

Bhaskar S. Kolachana, Richard C. Saunders, Daniel Weinberger

Research output: Contribution to journalArticle

Abstract

Extracellular gamma amino butyric acid (GABA) levels were measured in the caudate nucleus and the prefrontal cortex of the rhesus monkey brain using in vivo microdialysis under isofluorane gas anesthesia. Evoked GABA release was investigated for voltage sensitivity and calcium (Ca2+) dependency. There was a multifold increase in extracellular GABA levels following local perfusion with: (1) high potassium (50 mM, KCl), (2) veratridine (10 μ M), and (3) the GABA releasing agent and uptake blocker, (-) nipecotic acid (1 mM). Release of GABA was significantly reduced when veratridine or (-) nipecotic acid were coinfused in Ca2+-free cerebrospinal fluid (CSF). Coinfusion of nipecotic acid with TTX (10 μM) also resulted in attenuation of evoked GABA release. These results suggest that GABA levels recovered using in vivo microdialysis, from the caudate nucleus and the prefrontal cortex in the rhesus monkey, derive in significant part from vesicular pools and the exocytotic process is both Ca2+-dependent and voltage-sensitive.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalSynapse
Volume25
Issue number3
DOIs
StatePublished - Mar 1997
Externally publishedYes

Fingerprint

Butyric Acid
Caudate Nucleus
Prefrontal Cortex
Macaca mulatta
Veratridine
Microdialysis
Cerebrospinal Fluid
Potassium
Anesthesia
Perfusion
Gases
Calcium
Brain
nipecotic acid

Keywords

  • γ-aminobutyric acid
  • Ca dependency
  • caudate nucleus
  • high potassium
  • microdialysis
  • monkey
  • nipecotic acid
  • tetrodotoxin
  • veratridine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pharmacology

Cite this

In vivo characterization of extracellular GABA release in the caudate nucleus and prefrontal cortex of the rhesus monkey. / Kolachana, Bhaskar S.; Saunders, Richard C.; Weinberger, Daniel.

In: Synapse, Vol. 25, No. 3, 03.1997, p. 285-292.

Research output: Contribution to journalArticle

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