In vivo and in vitro release of ACTH by synthetic CRF

C. M. Turkelson, A. Arimura, M. D. Culler, J. B. Fishback, K. Groot, M. Kanda, M. Luciano, C. R. Thomas, D. Chang, J. K. Chang, M. Shimizu

Research output: Contribution to journalArticlepeer-review

Abstract

The 41-residue corticotropin releasing factor (CRF) was synthesized by the solid phase method. The synthetic CRF and arginine vasopressin (AVP) were examined for ACTH releasing activity and effects on the release of 5 other pituitary hormones in vivo and in vitro. Injection of the CRF into pharmacologically blocked rats increased plasma corticosterone levels in a dose-related manner. The minimum effective dose was 1.6×10-12 mol/100 g body weight. CRF also significantly stimulated release of ACTH-like immunoreactivity in a dose-related manner from rat pituitary quarters beginning at a concentration of 10-9 M. AVP, a peptide known to have CRF activity, exhibited slightly lower corticotropin releasing activity than the CRF at equimolar dose levels. Secretion of other pituitary hormones was not appreciably altered by either the CRF or AVP.

Original languageEnglish (US)
Pages (from-to)425-429
Number of pages5
JournalPeptides
Volume2
Issue number4
DOIs
StatePublished - 1981
Externally publishedYes

Keywords

  • Anterior pituitary hormones
  • Arginine vasopressin
  • Corticotropin releasing factor

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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