In-vivo and in-situ detection of atherosclerotic plaques using full-range complex-conjugate-free spectral domain optical coherence tomography in the murine carotid

Yong Huang, Robert Wicks, Kang Zhang, Mingtao Zhao, Betty M. Tyler, Lee Hwang, Gustavo Pradilla, Jin U. Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Carotid endarterectomy is a common vascular surgical procedure which may help prevent patients' risk of having a stroke. A high resolution real-time imaging technique that can detect the position and size of vascular plaques would provide great value to reduce the risk level and increase the surgical outcome. Optical coherence tomography (OCT), as a high resolution high speed noninvasive imaging technique, was evaluated in this study. Twenty-four 24-week old apolipoprotein E-deficient (ApoE-/-) mice were divided into three groups with 8 in each. One served as the control group fed with normal diet. One served as the study group fed with high-fat diet to induce atherosclerosis. The last served as the treatment group fed with both high-fat diet and medicine to treat atherosclerosis. Full-range, complex-conjugate-free spectral-domain OCT was used to image the mouse aorta near the neck area in-vivo with aorta exposed to the imaging head through surgical procedure. 2D and 3D images of the area of interest were presented real-time through graphics processing unit accelerated algorithm. In-situ imaging of all the mice after perfusion were performed again to validate the invivo detection result and to show potential capability of OCT if combined with surgical saline flush. Later all the imaged arteries were stained with H and E to perform histology analysis. Preliminary results confirmed the accuracy and fast imaging speed of OCT imaging technique in determining atherosclerosis.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics IX
DOIs
StatePublished - May 30 2013
EventPhotonic Therapeutics and Diagnostics IX - San Francisco, CA, United States
Duration: Feb 2 2013Feb 7 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8565
ISSN (Print)1605-7422

Other

OtherPhotonic Therapeutics and Diagnostics IX
CountryUnited States
CitySan Francisco, CA
Period2/2/132/7/13

Keywords

  • Atherosclerosis
  • Full-range optical coherence tomography
  • Neurosurgery

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Huang, Y., Wicks, R., Zhang, K., Zhao, M., Tyler, B. M., Hwang, L., Pradilla, G., & Kang, J. U. (2013). In-vivo and in-situ detection of atherosclerotic plaques using full-range complex-conjugate-free spectral domain optical coherence tomography in the murine carotid. In Photonic Therapeutics and Diagnostics IX [85654P] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8565). https://doi.org/10.1117/12.2001928