In vitro two-dimensional and three-dimensional tenocyte culture for tendon tissue engineering

Yiwei Qiu, Xiao Wang, Yaonan Zhang, Andrew J. Carr, Liwei Zhu, Zhidao Xia, Afsie Sabokbar

Research output: Contribution to journalArticle

Abstract

In order to examine the differentiation potential of the tenocytes expanded in our defined culture medium (reported previously) and the effect of sequential combination of the two culture conditions on human tenocytes, a two-dimensional and three-dimensional experimental approach was used. Human tenocytes were sequentially exposed to 1% fetal bovine serum (FBS)+50ng/ml platelet-derived growth factor-BB (PDGF BB ) + 50ng/ml basic fibroblast growth factor (bFGF) for the first 14days (expansion phase) followed by a further 14-day culture in the presence of 10ng/ml transforming growth factor β-3 plus 50ng/ml insulin-like growth factor 1, but in the absence of serum (differentiation phase). The results showed that by sequential treatment of human tenocytes maintaining a long-term two-dimensional tenocyte culture in vitro for up to 28days was possible. These findings were further verified using a three-dimensional scaffold (Bombyx silk) whereby the tendon-like constructs formed resembled macroscopically and microscopically the constructs formed in 10% FBS supplemented culture media and the human hamstring tendon. These findings were further substantiated using haematoxylin and eosin staining, scanning electron microscopy and by immunohistochemical detection of type I collagen. In addition, the mechanical properties of the three-dimensional constructs were determined to be significantly superior to that of the natural human hamstring tendon. This is the first report to demonstrate a possible approach in expanding and differentiating human tenocytes for tendon tissue engineering.

Original languageEnglish (US)
Pages (from-to)E216-E226
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume10
Issue number3
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Fingerprint

Tendons
Tissue Engineering
Tissue engineering
Culture Media
Silk
Insulin
Bioelectric potentials
Transforming Growth Factors
Somatomedins
Fibroblast Growth Factor 2
Fibroblasts
Hematoxylin
Eosine Yellowish-(YS)
Collagen Type I
Platelets
Serum
Collagen
Scaffolds
Bombyx
Electron Scanning Microscopy

Keywords

  • Growth factors
  • Scaffold
  • Sequential
  • Tendon
  • Tenocyte
  • Tissue engineering

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

In vitro two-dimensional and three-dimensional tenocyte culture for tendon tissue engineering. / Qiu, Yiwei; Wang, Xiao; Zhang, Yaonan; Carr, Andrew J.; Zhu, Liwei; Xia, Zhidao; Sabokbar, Afsie.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 10, No. 3, 01.03.2016, p. E216-E226.

Research output: Contribution to journalArticle

Qiu, Yiwei ; Wang, Xiao ; Zhang, Yaonan ; Carr, Andrew J. ; Zhu, Liwei ; Xia, Zhidao ; Sabokbar, Afsie. / In vitro two-dimensional and three-dimensional tenocyte culture for tendon tissue engineering. In: Journal of Tissue Engineering and Regenerative Medicine. 2016 ; Vol. 10, No. 3. pp. E216-E226.
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