In vitro transdifferentiation of bone marrow stromal cells into GABAergic-like neurons

Payam Mohammadgharibani, Taki Tiraihi, Jalil Arabkheradmand

Research output: Contribution to journalArticle

Abstract

Background: Cell therapy of many neurodegenerative diseases using bone marrow stromal cells (BMSC) requires the differentiation of BMSC into neuronal subtype. However, the transdifferentiation of BMSC into GABAergic phenotype requires more investigation. Methods: In this study, BMSC of adult female rats were pre-induced into neuroblast-like cells using 1 mM β-mercaptoethanol (βME) and 10 μM retinoic acid (RA), followed by 40 mM potassium chloride as inducer. The BMSC were evaluated by fibronectin as well as Oct-4. The percentage of nestin, neurofilaments (NF 68, NF 160, and NF 200) and GABA immuno-reactive cells was used to evaluate the GABAergic differentiation at the pre-induction and induction stages. The statistical analysis was carried out using unpaired student's t-test and ANOVA with Tukey's multiple comparison. Results: The BMSC in the fourth passage expressed fibronectin up to 91.24 ± 0.82%. The pre-induced cells after 2 days of RA exposure showed the expression of neuroblastic markers of nestin and NF68 (81.56 ± 2.64% and 82.12 ± 2.65%, respectively). The yield of GABAergic neurons with β-ME for 1 h and RA as preinducer for 2 days followed by potassium chloride as inducer (40 mM for 3 days) was 60.64% ± 1.97%. In addition, NF160 and NF200 were detected in the transdifferentiated cells. RT-PCR showed no expression of Oct-4 after the induction and pre-induction stages. Conclusion: GABAergic-like neurons obtained from BMSC can be potentially used in cell transplanting for some neurodegenerative disorders.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalIranian Biomedical Journal
Volume13
Issue number3
StatePublished - Jul 1 2009
Externally publishedYes

Fingerprint

GABAergic Neurons
Mesenchymal Stromal Cells
Neurons
Bone
Tretinoin
Nestin
Potassium Chloride
Mercaptoethanol
Fibronectins
Neurodegenerative Diseases
Neurodegenerative diseases
Intermediate Filaments
Cell- and Tissue-Based Therapy
Analysis of variance (ANOVA)
In Vitro Techniques
gamma-Aminobutyric Acid
Rats
Cell Differentiation
Statistical methods
Analysis of Variance

Keywords

  • Bone marrow stromal cells (BMSC)
  • Cell therapy
  • GABAergic-like neurons
  • Transdifferentiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

In vitro transdifferentiation of bone marrow stromal cells into GABAergic-like neurons. / Mohammadgharibani, Payam; Tiraihi, Taki; Arabkheradmand, Jalil.

In: Iranian Biomedical Journal, Vol. 13, No. 3, 01.07.2009, p. 137-143.

Research output: Contribution to journalArticle

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