Abstract
Background: Cell therapy of many neurodegenerative diseases using bone marrow stromal cells (BMSC) requires the differentiation of BMSC into neuronal subtype. However, the transdifferentiation of BMSC into GABAergic phenotype requires more investigation. Methods: In this study, BMSC of adult female rats were pre-induced into neuroblast-like cells using 1 mM β-mercaptoethanol (βME) and 10 μM retinoic acid (RA), followed by 40 mM potassium chloride as inducer. The BMSC were evaluated by fibronectin as well as Oct-4. The percentage of nestin, neurofilaments (NF 68, NF 160, and NF 200) and GABA immuno-reactive cells was used to evaluate the GABAergic differentiation at the pre-induction and induction stages. The statistical analysis was carried out using unpaired student's t-test and ANOVA with Tukey's multiple comparison. Results: The BMSC in the fourth passage expressed fibronectin up to 91.24 ± 0.82%. The pre-induced cells after 2 days of RA exposure showed the expression of neuroblastic markers of nestin and NF68 (81.56 ± 2.64% and 82.12 ± 2.65%, respectively). The yield of GABAergic neurons with β-ME for 1 h and RA as preinducer for 2 days followed by potassium chloride as inducer (40 mM for 3 days) was 60.64% ± 1.97%. In addition, NF160 and NF200 were detected in the transdifferentiated cells. RT-PCR showed no expression of Oct-4 after the induction and pre-induction stages. Conclusion: GABAergic-like neurons obtained from BMSC can be potentially used in cell transplanting for some neurodegenerative disorders.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 137-143 |
| Number of pages | 7 |
| Journal | Iranian Biomedical Journal |
| Volume | 13 |
| Issue number | 3 |
| State | Published - Jul 1 2009 |
| Externally published | Yes |
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Keywords
- Bone marrow stromal cells (BMSC)
- Cell therapy
- GABAergic-like neurons
- Transdifferentiation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Biochemistry, medical
Cite this
In vitro transdifferentiation of bone marrow stromal cells into GABAergic-like neurons. / Mohammadgharibani, Payam; Tiraihi, Taki; Arabkheradmand, Jalil.
In: Iranian Biomedical Journal, Vol. 13, No. 3, 01.07.2009, p. 137-143.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro transdifferentiation of bone marrow stromal cells into GABAergic-like neurons
AU - Mohammadgharibani, Payam
AU - Tiraihi, Taki
AU - Arabkheradmand, Jalil
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Background: Cell therapy of many neurodegenerative diseases using bone marrow stromal cells (BMSC) requires the differentiation of BMSC into neuronal subtype. However, the transdifferentiation of BMSC into GABAergic phenotype requires more investigation. Methods: In this study, BMSC of adult female rats were pre-induced into neuroblast-like cells using 1 mM β-mercaptoethanol (βME) and 10 μM retinoic acid (RA), followed by 40 mM potassium chloride as inducer. The BMSC were evaluated by fibronectin as well as Oct-4. The percentage of nestin, neurofilaments (NF 68, NF 160, and NF 200) and GABA immuno-reactive cells was used to evaluate the GABAergic differentiation at the pre-induction and induction stages. The statistical analysis was carried out using unpaired student's t-test and ANOVA with Tukey's multiple comparison. Results: The BMSC in the fourth passage expressed fibronectin up to 91.24 ± 0.82%. The pre-induced cells after 2 days of RA exposure showed the expression of neuroblastic markers of nestin and NF68 (81.56 ± 2.64% and 82.12 ± 2.65%, respectively). The yield of GABAergic neurons with β-ME for 1 h and RA as preinducer for 2 days followed by potassium chloride as inducer (40 mM for 3 days) was 60.64% ± 1.97%. In addition, NF160 and NF200 were detected in the transdifferentiated cells. RT-PCR showed no expression of Oct-4 after the induction and pre-induction stages. Conclusion: GABAergic-like neurons obtained from BMSC can be potentially used in cell transplanting for some neurodegenerative disorders.
AB - Background: Cell therapy of many neurodegenerative diseases using bone marrow stromal cells (BMSC) requires the differentiation of BMSC into neuronal subtype. However, the transdifferentiation of BMSC into GABAergic phenotype requires more investigation. Methods: In this study, BMSC of adult female rats were pre-induced into neuroblast-like cells using 1 mM β-mercaptoethanol (βME) and 10 μM retinoic acid (RA), followed by 40 mM potassium chloride as inducer. The BMSC were evaluated by fibronectin as well as Oct-4. The percentage of nestin, neurofilaments (NF 68, NF 160, and NF 200) and GABA immuno-reactive cells was used to evaluate the GABAergic differentiation at the pre-induction and induction stages. The statistical analysis was carried out using unpaired student's t-test and ANOVA with Tukey's multiple comparison. Results: The BMSC in the fourth passage expressed fibronectin up to 91.24 ± 0.82%. The pre-induced cells after 2 days of RA exposure showed the expression of neuroblastic markers of nestin and NF68 (81.56 ± 2.64% and 82.12 ± 2.65%, respectively). The yield of GABAergic neurons with β-ME for 1 h and RA as preinducer for 2 days followed by potassium chloride as inducer (40 mM for 3 days) was 60.64% ± 1.97%. In addition, NF160 and NF200 were detected in the transdifferentiated cells. RT-PCR showed no expression of Oct-4 after the induction and pre-induction stages. Conclusion: GABAergic-like neurons obtained from BMSC can be potentially used in cell transplanting for some neurodegenerative disorders.
KW - Bone marrow stromal cells (BMSC)
KW - Cell therapy
KW - GABAergic-like neurons
KW - Transdifferentiation
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UR - http://www.scopus.com/inward/citedby.url?scp=69249129125&partnerID=8YFLogxK
M3 - Article
C2 - 19688019
AN - SCOPUS:69249129125
VL - 13
SP - 137
EP - 143
JO - Iranian Biomedical Journal
JF - Iranian Biomedical Journal
SN - 1028-852X
IS - 3
ER -