In vitro synthesis of RNA with "aggregate" enzyme, chromatin, and DNA from liver of methylazoxymethanol acetate-treated rats

Dennis J. Grab, Morris S. Zedeck, Norbert I. Swislocki, Martin Sonenberg

Research output: Contribution to journalArticle

Abstract

"Aggregate" enzyme, chromatin and DNA preparations were isolated from livers of rats treated with the carcinogen, methylazoxymethanol (MAM) acetate. DNA template activity for RNA synthesis in vitro was unimpaired while the template activity of chromatin was slightly reduced. There was a marked inhibition of UTP incorporation into RNA, however, when the "aggregate" enzyme preparation was the source of both template and RNA polymerase. Circular dichroism analysis of the "aggregate" enzyme preparation indicated a change in conformation of the protein component. The results suggest that MAM acetate interacts with nuclear proteins and produces conformational changes which result in a decreased RNA synthesis.

Original languageEnglish (US)
Pages (from-to)259-267
Number of pages9
JournalChemico-Biological Interactions
Volume6
Issue number4
DOIs
StatePublished - 1973
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology

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