TY - JOUR
T1 - In vitro synthesis of RNA with "aggregate" enzyme, chromatin, and DNA from liver of methylazoxymethanol acetate-treated rats
AU - Grab, Dennis J.
AU - Zedeck, Morris S.
AU - Swislocki, Norbert I.
AU - Sonenberg, Martin
PY - 1973
Y1 - 1973
N2 - "Aggregate" enzyme, chromatin and DNA preparations were isolated from livers of rats treated with the carcinogen, methylazoxymethanol (MAM) acetate. DNA template activity for RNA synthesis in vitro was unimpaired while the template activity of chromatin was slightly reduced. There was a marked inhibition of UTP incorporation into RNA, however, when the "aggregate" enzyme preparation was the source of both template and RNA polymerase. Circular dichroism analysis of the "aggregate" enzyme preparation indicated a change in conformation of the protein component. The results suggest that MAM acetate interacts with nuclear proteins and produces conformational changes which result in a decreased RNA synthesis.
AB - "Aggregate" enzyme, chromatin and DNA preparations were isolated from livers of rats treated with the carcinogen, methylazoxymethanol (MAM) acetate. DNA template activity for RNA synthesis in vitro was unimpaired while the template activity of chromatin was slightly reduced. There was a marked inhibition of UTP incorporation into RNA, however, when the "aggregate" enzyme preparation was the source of both template and RNA polymerase. Circular dichroism analysis of the "aggregate" enzyme preparation indicated a change in conformation of the protein component. The results suggest that MAM acetate interacts with nuclear proteins and produces conformational changes which result in a decreased RNA synthesis.
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U2 - 10.1016/0009-2797(73)90053-7
DO - 10.1016/0009-2797(73)90053-7
M3 - Article
C2 - 4574255
AN - SCOPUS:0015608364
SN - 0009-2797
VL - 6
SP - 259
EP - 267
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 4
ER -