In vitro synthesis of proteins in bacterial extracts

Hani S. Zaher, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol describes the methods used to generate protein in a cell-free system derived from E. coli. The in vitro synthesis of protein has been used in studying many ribosome-based gene regulation steps (Gong and Yanofsky, 2001). Such techniques have also been used to study protein-protein, protein-DNA, and protein-RNA interactions, and to produce radiolabeled protein species. More recently, such approaches have been utilized to produce large quantities of toxic proteins for proteomic and structural studies (Yokoyama et al., 2000).

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part B
PublisherAcademic Press Inc.
Pages3-15
Number of pages13
ISBN (Print)9780124201200
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume539
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Zaher, H. S., & Green, R. (2014). In vitro synthesis of proteins in bacterial extracts. In Laboratory Methods in Enzymology: Protein Part B (pp. 3-15). (Methods in Enzymology; Vol. 539). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420120-0.00001-3