In vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against Listeria monocytogenes

Noton Dutta, K. Mazumdar, Jae H. Park

Research output: Contribution to journalArticle

Abstract

A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro. Methods and Results: The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0·37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant (P < 0·05) for Dc plus Gm but not Dc plus Am. Conclusions: Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis. Significance and Impact of the Study: Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs.

Original languageEnglish (US)
Pages (from-to)783-785
Number of pages3
JournalLetters in Applied Microbiology
Volume48
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

Fingerprint

Diclofenac
Listeria monocytogenes
Gentamicins
Anti-Inflammatory Agents
Ampicillin
Listeriosis
Anti-Bacterial Agents
Pharmaceutical Preparations
In Vitro Techniques
Therapeutics

Keywords

  • Diclofenac
  • Gentamicin
  • Listeria monocytogenes
  • Synergism

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

In vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against Listeria monocytogenes. / Dutta, Noton; Mazumdar, K.; Park, Jae H.

In: Letters in Applied Microbiology, Vol. 48, No. 6, 06.2009, p. 783-785.

Research output: Contribution to journalArticle

@article{9a966b3f2c8d4440b373d4847cf53bd9,
title = "In vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against Listeria monocytogenes",
abstract = "A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro. Methods and Results: The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0·37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant (P < 0·05) for Dc plus Gm but not Dc plus Am. Conclusions: Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis. Significance and Impact of the Study: Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs.",
keywords = "Diclofenac, Gentamicin, Listeria monocytogenes, Synergism",
author = "Noton Dutta and K. Mazumdar and Park, {Jae H.}",
year = "2009",
month = "6",
doi = "10.1111/j.1472-765X.2009.02588.x",
language = "English (US)",
volume = "48",
pages = "783--785",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - In vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against Listeria monocytogenes

AU - Dutta, Noton

AU - Mazumdar, K.

AU - Park, Jae H.

PY - 2009/6

Y1 - 2009/6

N2 - A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro. Methods and Results: The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0·37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant (P < 0·05) for Dc plus Gm but not Dc plus Am. Conclusions: Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis. Significance and Impact of the Study: Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs.

AB - A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro. Methods and Results: The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0·37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant (P < 0·05) for Dc plus Gm but not Dc plus Am. Conclusions: Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis. Significance and Impact of the Study: Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs.

KW - Diclofenac

KW - Gentamicin

KW - Listeria monocytogenes

KW - Synergism

UR - http://www.scopus.com/inward/record.url?scp=65649124214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65649124214&partnerID=8YFLogxK

U2 - 10.1111/j.1472-765X.2009.02588.x

DO - 10.1111/j.1472-765X.2009.02588.x

M3 - Article

C2 - 19291208

AN - SCOPUS:65649124214

VL - 48

SP - 783

EP - 785

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 6

ER -