In vitro studies of isolated supported human hearts

Daniel Burkhoff, John T. Flaherty, David T. Yue, Ahvie Herskowitz, Robert Y. Oikawa, Seiryo Sugiura, Michael R. Franz, William A. Baumgartner, Jochen Schaefer, Bruce A. Reitz, Kiichi Sagawa

Research output: Contribution to journalArticlepeer-review

Abstract

We developed methods to revive human hearts, obtained at the time of cardiac transplantation, and study them in the physiology laboratory. The hearts were arrested with cardioplegic solution at the time of explantation and transported to the laboratory at 4°C. The hearts were perfused with a human blood based solution whose flow rate, temperature, and ionic concentration were controlled. Six hearts with various endstage cardiomyopathies were revived in this manner. Once perfusion was started, the hearts maintained a steady contractile state for approximately 30 min during which time data could be collected. Within this time period we could measure end-systolic and end-diastolic pressure-volume relations, the time courses of contraction and relaxation, and the influence of heart rate and premature stimulation on contractile state. The results suggest that evidence of specific cellular abnormalities in human heart disease might be obtained from measurements of global ventricular performance. Furthermore, the type of abnormality identified, namely sarcoplasmic reticulum dysfunction, in several forms of cardiomyopathy was in concordance with results obtained in muscle bath studies of similarly diseased human and animal myocardium.

Original languageEnglish (US)
Pages (from-to)185-196
Number of pages12
JournalHeart and Vessels
Volume4
Issue number4
DOIs
StatePublished - Dec 1 1988

Keywords

  • Cardiomyopathy
  • End-diastolic pressure-volume relationship
  • End-systolic pressure-volume relationship
  • Force-interval relationship
  • Heart rate
  • Relaxation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'In vitro studies of isolated supported human hearts'. Together they form a unique fingerprint.

Cite this