In vitro stimulation by paraquat of reactive oxygen-mediated lipid peroxidation in rat lung microsomes

Michael A. Trush, Edward G. Mimnaugh, Erika Ginsburg, Theodore E. Gram

Research output: Contribution to journalArticle

Abstract

Paraquat significantly stimulated lipid peroxidation in rat lung microsomes without the addition of exogenous iron. The ability of paraquat to stimulate this lipid peroxidation was dependent on the presence of adequate reducing equivalents (NADPH), aerobic conditions, and the duration of incubation, viz. optimal in vitro conditions. Even greater paraquat-mediated lipid peroxidation was observed if incubations were conducted under O2 or if vitamin E-deficient microsomes were utilized, factors which have previously been reported to increase the in vivo pulnonary toxicity of paraquat. Superoxide dismutase (3 μg/ml) significantly inhibited paraquat-stimulated lipid peroxidation in rat lung microsomes (73%), demonstrating a pivotal role for superoxide in this process. Thus, the redox cycling of paraquat and accompanying reactive oxygen generation was capable of mediating lipid peroxidation not only in mouse lung and rat liver microsomes but also in rat lung microsomes.

Original languageEnglish (US)
Pages (from-to)279-286
Number of pages8
JournalToxicology and Applied Pharmacology
Volume60
Issue number2
DOIs
StatePublished - Sep 15 1981

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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