In Vitro Sensitivity of Measles Virus to 6-Azauridine

Larry L. Leonard; Volker Ter Meulen; John M. Freeman

doi:10.3181/00379727-136-35380

In Vitro Sensitivity of Measles Virus to 6-Azauridine

Larry L. Leonard, Volker Ter Meulen, John M. Freeman

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The effect of 6-azauridine, an inhibitor of RNA synthesis, has been shown by 2 experimental methods to inhibit the replication of measles virus within mammalian cells in vitro. In a direct virus titration in which the drug was incorporated in the agar overlay, 6-AZ not only reduced the infectivity of measles virus but also delayed plaque development. Experiments designed to test the effectiveness of 6-azauridine when added to cultures at intervals after infection, showed that this uridine analog was capable of reducing the amount of infectious virus produced by each infected cell, and that the drug is more effective when added early in the infection cycle.

Original language	English (US)
Pages (from-to)	857-862
Number of pages	6
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	136
Issue number	3
DOIs	https://doi.org/10.3181/00379727-136-35380
State	Published - Mar 1971
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "In Vitro Sensitivity of Measles Virus to 6-Azauridine",

abstract = "The effect of 6-azauridine, an inhibitor of RNA synthesis, has been shown by 2 experimental methods to inhibit the replication of measles virus within mammalian cells in vitro. In a direct virus titration in which the drug was incorporated in the agar overlay, 6-AZ not only reduced the infectivity of measles virus but also delayed plaque development. Experiments designed to test the effectiveness of 6-azauridine when added to cultures at intervals after infection, showed that this uridine analog was capable of reducing the amount of infectious virus produced by each infected cell, and that the drug is more effective when added early in the infection cycle.",

author = "Leonard, {Larry L.} and Meulen, {Volker Ter} and Freeman, {John M.}",

note = "Funding Information: This study was supported by Grant AI-01475 from the National Institute of Allergy and Infectious Diseases, National Institute of Health, U.S. Public Health Service, Department of Health, Education and Welfare, and also in part by the “Deutsche Forschungsgemeinschaft,” AZ: Me 270/4 and Me 270/6. 2 ",

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doi = "10.3181/00379727-136-35380",

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AU - Meulen, Volker Ter

AU - Freeman, John M.

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N2 - The effect of 6-azauridine, an inhibitor of RNA synthesis, has been shown by 2 experimental methods to inhibit the replication of measles virus within mammalian cells in vitro. In a direct virus titration in which the drug was incorporated in the agar overlay, 6-AZ not only reduced the infectivity of measles virus but also delayed plaque development. Experiments designed to test the effectiveness of 6-azauridine when added to cultures at intervals after infection, showed that this uridine analog was capable of reducing the amount of infectious virus produced by each infected cell, and that the drug is more effective when added early in the infection cycle.

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In Vitro Sensitivity of Measles Virus to 6-Azauridine

Abstract

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