In vitro reversal of indomethacin-blocked ovulation by prostaglandin F(2α)

Y. Hamada, K. H. Wright, E. E. Wallach

Research output: Contribution to journalArticlepeer-review


An in vitro perfused ovary preparation was used to study the role of prostaglandin F(2α) (PGF(2α))in follicle rupture. The administration of PGF(2α) alone has been shown to restore indomethacin-blocked ovulation in rabbits and monkeys. In the model used, ovulation consistently occurred when human chorionic gonadotropin (hCG) was given to the intact rabbit prior to ovarian removal. hCG-induced ovulation was blocked in both perfused and in situ control ovaries by indomethacin (10 mg/kg IV 6 hr after hCG) given to the intact animal. The addition of PGF(2α)(1 mg/200 ml) to the perfusion fluid restored ovulation in the isolated ovary as compared with the in situ ovary (P<0.005) and with the perfused, untreated ovary (P<0.01). Following removal and perfusion of both ovaries from rabbits treated with indomethacin, ovulation occurred following the addition of PGF(2α) to the perfusate, but did not occur without PGF(2α) (P<0.05). These data indicate that indomethacin can block ovulation and that ovulation can be restored by the addition of PGF(2α) to the perfusion system, further supporting the significance of PGF(2α) in the process of follicular rupture.

Original languageEnglish (US)
Pages (from-to)702-706
Number of pages5
JournalFertility and sterility
Issue number6
StatePublished - 1978
Externally publishedYes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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