Abstract
Background: The CRISPR-Cas immune system protects E. coli against invasive DNA. Results: We have reconstituted this immune system in vitro using recombinant proteins. Conclusion: Degradation of invasive DNA is tightly regulated and unidirectional. Significance: Reconstitution provides an invaluable tool for understanding the CRISPR-Cas immune system.
Original language | English (US) |
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Pages (from-to) | 22184-22192 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 31 |
DOIs | |
State | Published - Aug 2 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology