In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA Target

Sabin Mulepati, Scott Bailey

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The CRISPR-Cas immune system protects E. coli against invasive DNA. Results: We have reconstituted this immune system in vitro using recombinant proteins. Conclusion: Degradation of invasive DNA is tightly regulated and unidirectional. Significance: Reconstitution provides an invaluable tool for understanding the CRISPR-Cas immune system.

Original languageEnglish (US)
Pages (from-to)22184-22192
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number31
DOIs
StatePublished - Aug 2 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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