In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA Target

Sabin Mulepati; Scott Bailey

doi:10.1074/jbc.M113.472233

In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA Target

Sabin Mulepati, Scott Bailey

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

Background: The CRISPR-Cas immune system protects E. coli against invasive DNA. Results: We have reconstituted this immune system in vitro using recombinant proteins. Conclusion: Degradation of invasive DNA is tightly regulated and unidirectional. Significance: Reconstitution provides an invaluable tool for understanding the CRISPR-Cas immune system.

Original language	English (US)
Pages (from-to)	22184-22192
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	31
DOIs	https://doi.org/10.1074/jbc.M113.472233
State	Published - Aug 2 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.M113.472233

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abstract = "Background: The CRISPR-Cas immune system protects E. coli against invasive DNA. Results: We have reconstituted this immune system in vitro using recombinant proteins. Conclusion: Degradation of invasive DNA is tightly regulated and unidirectional. Significance: Reconstitution provides an invaluable tool for understanding the CRISPR-Cas immune system.",

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In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA Target

Abstract

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