In vitro production of Panton-Valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections

Stephanie M. Hamilton, Amy E. Bryant, Karen C Carroll, Vivian Lockary, Yongsheng Ma, Eric McIndoo, Loren G. Miller, Francoise Perdreau-Remington, John Pullman, George F. Risi, Daniel B. Salmi, Dennis L. Stevens

Research output: Contribution to journalArticle

Abstract

Background. Community-acquired methicillin-resistant Staphylococcus aureus strains have recently been associated with severe necrotizing infections. Greater than 75% of these strains carry the genes for Panton-Valentine leukocidin (PVL), suggesting that this toxin may mediate these severe infections. However, to date, studies have not provided evidence of toxin production. Methods. Twenty-nine community-acquired methicillin-resistant Staphylococcus aureus and 2 community-acquired methicillin-susceptible S. aureus strains were collected from patients with infections of varying severity. Strains were analyzed for the presence of lukF-PV and SCCmecA type. PVL production in lukF-PV gene-positive strains was measured by ELISA, and the amount produced was analyzed relative to severity of infection. Results. Only 2 of the 31 strains tested, 1 methicillin-resistant Staphylococcus aureus abscess isolate and 1 nasal carriage methicillin-susceptible S. aureus isolate, were lukF-PV negative. All methicillin-resistant Staphylococcus aureus strains were SCCmec type IV. PVL was produced by all strains harboring lukF-PV, although a marked strain-to-strain variation was observed. Twenty-six (90%) of 29 strains produced 50-350 ng/mL of PVL; the remaining strains produced PVL in excess of 500 ng/mL. The quantity of PVL produced in vitro did not correlate with severity of infection. Conclusions. Although PVL likely plays an important role in the pathogenesis of these infections, its mere presence is not solely responsible for the increased severity. Factors that up-regulate toxin synthesis in vivo could contribute to more-severe disease and worse outcomes in patients with community-acquired methicillin-resistant Staphylococcus aureus infection.

Original languageEnglish (US)
Pages (from-to)1550-1558
Number of pages9
JournalClinical Infectious Diseases
Volume45
Issue number12
DOIs
StatePublished - Dec 15 2007

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Methicillin-Resistant Staphylococcus aureus
Infection
Methicillin
Staphylococcus aureus
Panton-Valentine leukocidin
In Vitro Techniques
Nose
Abscess
Genes
Up-Regulation
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

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In vitro production of Panton-Valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections. / Hamilton, Stephanie M.; Bryant, Amy E.; Carroll, Karen C; Lockary, Vivian; Ma, Yongsheng; McIndoo, Eric; Miller, Loren G.; Perdreau-Remington, Francoise; Pullman, John; Risi, George F.; Salmi, Daniel B.; Stevens, Dennis L.

In: Clinical Infectious Diseases, Vol. 45, No. 12, 15.12.2007, p. 1550-1558.

Research output: Contribution to journalArticle

Hamilton, SM, Bryant, AE, Carroll, KC, Lockary, V, Ma, Y, McIndoo, E, Miller, LG, Perdreau-Remington, F, Pullman, J, Risi, GF, Salmi, DB & Stevens, DL 2007, 'In vitro production of Panton-Valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections', Clinical Infectious Diseases, vol. 45, no. 12, pp. 1550-1558. https://doi.org/10.1086/523581
Hamilton, Stephanie M. ; Bryant, Amy E. ; Carroll, Karen C ; Lockary, Vivian ; Ma, Yongsheng ; McIndoo, Eric ; Miller, Loren G. ; Perdreau-Remington, Francoise ; Pullman, John ; Risi, George F. ; Salmi, Daniel B. ; Stevens, Dennis L. / In vitro production of Panton-Valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections. In: Clinical Infectious Diseases. 2007 ; Vol. 45, No. 12. pp. 1550-1558.
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abstract = "Background. Community-acquired methicillin-resistant Staphylococcus aureus strains have recently been associated with severe necrotizing infections. Greater than 75{\%} of these strains carry the genes for Panton-Valentine leukocidin (PVL), suggesting that this toxin may mediate these severe infections. However, to date, studies have not provided evidence of toxin production. Methods. Twenty-nine community-acquired methicillin-resistant Staphylococcus aureus and 2 community-acquired methicillin-susceptible S. aureus strains were collected from patients with infections of varying severity. Strains were analyzed for the presence of lukF-PV and SCCmecA type. PVL production in lukF-PV gene-positive strains was measured by ELISA, and the amount produced was analyzed relative to severity of infection. Results. Only 2 of the 31 strains tested, 1 methicillin-resistant Staphylococcus aureus abscess isolate and 1 nasal carriage methicillin-susceptible S. aureus isolate, were lukF-PV negative. All methicillin-resistant Staphylococcus aureus strains were SCCmec type IV. PVL was produced by all strains harboring lukF-PV, although a marked strain-to-strain variation was observed. Twenty-six (90{\%}) of 29 strains produced 50-350 ng/mL of PVL; the remaining strains produced PVL in excess of 500 ng/mL. The quantity of PVL produced in vitro did not correlate with severity of infection. Conclusions. Although PVL likely plays an important role in the pathogenesis of these infections, its mere presence is not solely responsible for the increased severity. Factors that up-regulate toxin synthesis in vivo could contribute to more-severe disease and worse outcomes in patients with community-acquired methicillin-resistant Staphylococcus aureus infection.",
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T1 - In vitro production of Panton-Valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections

AU - Hamilton, Stephanie M.

AU - Bryant, Amy E.

AU - Carroll, Karen C

AU - Lockary, Vivian

AU - Ma, Yongsheng

AU - McIndoo, Eric

AU - Miller, Loren G.

AU - Perdreau-Remington, Francoise

AU - Pullman, John

AU - Risi, George F.

AU - Salmi, Daniel B.

AU - Stevens, Dennis L.

PY - 2007/12/15

Y1 - 2007/12/15

N2 - Background. Community-acquired methicillin-resistant Staphylococcus aureus strains have recently been associated with severe necrotizing infections. Greater than 75% of these strains carry the genes for Panton-Valentine leukocidin (PVL), suggesting that this toxin may mediate these severe infections. However, to date, studies have not provided evidence of toxin production. Methods. Twenty-nine community-acquired methicillin-resistant Staphylococcus aureus and 2 community-acquired methicillin-susceptible S. aureus strains were collected from patients with infections of varying severity. Strains were analyzed for the presence of lukF-PV and SCCmecA type. PVL production in lukF-PV gene-positive strains was measured by ELISA, and the amount produced was analyzed relative to severity of infection. Results. Only 2 of the 31 strains tested, 1 methicillin-resistant Staphylococcus aureus abscess isolate and 1 nasal carriage methicillin-susceptible S. aureus isolate, were lukF-PV negative. All methicillin-resistant Staphylococcus aureus strains were SCCmec type IV. PVL was produced by all strains harboring lukF-PV, although a marked strain-to-strain variation was observed. Twenty-six (90%) of 29 strains produced 50-350 ng/mL of PVL; the remaining strains produced PVL in excess of 500 ng/mL. The quantity of PVL produced in vitro did not correlate with severity of infection. Conclusions. Although PVL likely plays an important role in the pathogenesis of these infections, its mere presence is not solely responsible for the increased severity. Factors that up-regulate toxin synthesis in vivo could contribute to more-severe disease and worse outcomes in patients with community-acquired methicillin-resistant Staphylococcus aureus infection.

AB - Background. Community-acquired methicillin-resistant Staphylococcus aureus strains have recently been associated with severe necrotizing infections. Greater than 75% of these strains carry the genes for Panton-Valentine leukocidin (PVL), suggesting that this toxin may mediate these severe infections. However, to date, studies have not provided evidence of toxin production. Methods. Twenty-nine community-acquired methicillin-resistant Staphylococcus aureus and 2 community-acquired methicillin-susceptible S. aureus strains were collected from patients with infections of varying severity. Strains were analyzed for the presence of lukF-PV and SCCmecA type. PVL production in lukF-PV gene-positive strains was measured by ELISA, and the amount produced was analyzed relative to severity of infection. Results. Only 2 of the 31 strains tested, 1 methicillin-resistant Staphylococcus aureus abscess isolate and 1 nasal carriage methicillin-susceptible S. aureus isolate, were lukF-PV negative. All methicillin-resistant Staphylococcus aureus strains were SCCmec type IV. PVL was produced by all strains harboring lukF-PV, although a marked strain-to-strain variation was observed. Twenty-six (90%) of 29 strains produced 50-350 ng/mL of PVL; the remaining strains produced PVL in excess of 500 ng/mL. The quantity of PVL produced in vitro did not correlate with severity of infection. Conclusions. Although PVL likely plays an important role in the pathogenesis of these infections, its mere presence is not solely responsible for the increased severity. Factors that up-regulate toxin synthesis in vivo could contribute to more-severe disease and worse outcomes in patients with community-acquired methicillin-resistant Staphylococcus aureus infection.

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