In vitro pituitary hormone releasing activity of 40 residue human pancreatic tumor growth hormone releasing factor

A. Arimura, M. D. Culler, C. M. Turkelson, M. G. Luciano, C. R. Thomas, N. Obara, K. Groot, J. Rivier, W. Vale

Research output: Contribution to journalArticlepeer-review

Abstract

The hypophysiotropic activities of a synthetic human pancreatic growth hormone releasing factor (hpGRF) with 40 residues was examined in vitro using rat pituitary halves. At concentrations from 10-10 M to 10-7 M the peptide stimulated GH release in a dose-dependent manner with the ED50 being 1.2×10-9 M. The concentration of 10-10 M hpGRF is comparable to the basal hypophyseal portal blood levels of other known hypothalamic hypophysiotropic hormones. However, GH release was enhanced three-fold by concentration as low as 10-12 M, though no dose-response relationship was observed up to 10-10 M. Thus, this peptide not only stimulates the release of GH in a dose-dependent manner, but at lower concentrations also maintains elevated GH levels. The release of ACTH, β-endorphin, LH, and FSH was not affected by hpGRF at any of the concentrations tested. At hpGRF concentrations less than 10-7 M, the release of TSH and PRL were unaffected. However, at 10-6 M, TSH release was enhanced about 2.5 fold and prolactin release was elevated slightly.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalPeptides
Volume4
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • GH Pituitary hormones
  • Growth hormone releasing factor (GRF)
  • In vitro test
  • Rat pituitary

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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