In vitro phagocytosis af Giardia duodenalis cysts by hemocytes of the Asian freshwater clam Corbicula fluminea

Thaddeus K. Graczyk, Michael R. Cranfield, David Bruce Conn

Research output: Contribution to journalArticle

Abstract

Hemocytes of the Asian freshwater clam Corbicula fluminea, phagocytosed in vitro infectious Giardia duodenalis cysts. After 15, 30, 60, 90, and 120 min of incubation an average of 22%, 32%, 43%, 54%, and 72% of the cysts were phagocytosed by 22%, 55%, 63%, 81%, and 86% of the hemocytes, respectively. The number of hemocytes showing phagocytosis and the mean number of cysts ingested per hemocyte increased significantly over time (P <0.01); the numbers of nonphagocytosed cysts significantly decreased (P <0.02). Extrapolation reveals that C. fluminea can retain by phagocytosis an average of 1.6 x 106 G. duodenalis cysts/ml hemolymph. The phagocytic capacity of C. fluminea hemocytes indicates the applicability of this freshwater benthic bivalve for bioindication of contamination of waste waters and agricultural drainage with Giardia cysts.

Original languageEnglish (US)
Pages (from-to)743-745
Number of pages3
JournalParasitology Research
Volume83
Issue number8
DOIs
StatePublished - 1997

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Corbicula
Corbicula fluminea
Hemocytes
Giardia lamblia
Bivalvia
hemocytes
Fresh Water
phagocytosis
clams
Phagocytosis
Cysts
Giardia
Hemolymph
hemolymph
wastewater
Waste Water
drainage
In Vitro Techniques
Drainage

ASJC Scopus subject areas

  • Parasitology

Cite this

In vitro phagocytosis af Giardia duodenalis cysts by hemocytes of the Asian freshwater clam Corbicula fluminea. / Graczyk, Thaddeus K.; Cranfield, Michael R.; Conn, David Bruce.

In: Parasitology Research, Vol. 83, No. 8, 1997, p. 743-745.

Research output: Contribution to journalArticle

Graczyk, Thaddeus K. ; Cranfield, Michael R. ; Conn, David Bruce. / In vitro phagocytosis af Giardia duodenalis cysts by hemocytes of the Asian freshwater clam Corbicula fluminea. In: Parasitology Research. 1997 ; Vol. 83, No. 8. pp. 743-745.
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