Abstract
The differentiation of epithelial tissue in organ cultures of murine buccal mucosa, various human oral mucosa, and human newborn foreskin was found to be dependent on the calcium concentration of the culture media. In low calcium medium (≤0.07 m M) epithelial differentiation was inhibited. The original stratifying layers separate and can be removed, producing a destratified explant. Histologically such an explant consits of a dorsal epithelial layer of basal keratinocytes resting on an intact basal lamina with subjacent stroma. At 0.01 m M calcium, the epithelial layer was one to two cells thick whereas at 0.07 m M it could be three or more layers in thickness with the most superficial cells being spread over the underlying cells. In addition to differentiation, keratinocyte migration over the sides of the explant (epiboly) and epithelial proliferation as determined by [3H]thymidine autoradiography were reduced by culture in low calcium medium. Redifferentiation occurs upon return to normal calcium levels (1.8 m M); addition of hydrocortisone to low calcium media was found to facilitate this redifferentiation.
Original language | English (US) |
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Pages (from-to) | 99-107 |
Number of pages | 9 |
Journal | In Vitro Cellular & Developmental Biology |
Volume | 21 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1985 |
Externally published | Yes |
Keywords
- calcium
- differentiation
- epithelium
- mucosa
- organ cultures
- proliferation
ASJC Scopus subject areas
- Embryology
- Developmental Biology
- Cell Biology
- Plant Science
- Biotechnology
- Clinical Biochemistry