TY - JOUR
T1 - In vitro metastatic colonization of human ovarian cancer cells to the omentum
AU - Khan, Shaheena M.
AU - Funk, Holly M.
AU - Thiolloy, Sophie
AU - Lotan, Tamara L.
AU - Hickson, Jonathan
AU - Prins, Gail S.
AU - Drew, Angela F.
AU - Rinker-Schaeffer, Carrie W.
N1 - Funding Information:
Acknowledgments We would like to thank Ms. Jennifer Taylor for her support and assistance in the conducting the intraperitoneal injections and for generously providing the SKOV3ip.1-GFP tagged cells for use in this study. We appreciate the supportive and enthusiastic input of Dr. Karl Matlin and Dr. Jerry Turner of the University of Chicago as we developed work reported herein. We appreciate the superb technical support and assistance of Dr. Lynnette Gerhold of the University of Chicago Optical Imaging Core Facility and Ms. Shirley Bond of the University of Chicago Biological Sciences Division Microscopy Core Facility. This work was made possible by the generous philanthropic support of The University of Chicago Section of Urology and grants from The Department of Defense Ovarian Cancer Research Program (W81XWH-09-0127) and the National Cancer Institute (2R01CA089569-06A2).
PY - 2010/3
Y1 - 2010/3
N2 - Despite the potentially crucial contributions of the omentum in the regulation of ovarian cancer metastatic growth, it remains a poorly understood organ. Due to its anatomic location and structural fragility, the omentum presents inherent challenges to mechanism-based in vivo studies. Thus, the availability of an ex vivo omental model would, in part, address some of these difficulties posed. Here we describe a technique for identifying, isolating and maintaining ex vivo cultures of omenta from immune-compromised and -competent mice. Ex vivo culture conditions were developed that maintain tissue viability, architecture, and function for up to 10 days. Further experiments demonstrate that the ex vivo culture conditions allow for the proliferation of ovarian cancer cells in vitro and support a similar pattern of microscopic lesions after either intraperitoneal injection of ovarian cancer cells or co-culture of ovarian cancer cells with the omentum. In agreement with previous studies from our laboratory, histologic evaluation of these specimens found that ovarian cancer cells, as well as other peritoneal cancer cells, preferentially accumulate in, and colonize, omental areas rich in immune cells. We now recognize that these are specific, functional structures referred to as milky spots. In sum, these are foundational studies of a readily accessible model, which is easily manipulated and can be immediately used to study the dynamic process of omental colonization. It is hoped that investigators will use the data herein as a starting point for refinements and modifications which will enable them to tailor the model to the specific needs of the experimental question(s) they wish to pursue.
AB - Despite the potentially crucial contributions of the omentum in the regulation of ovarian cancer metastatic growth, it remains a poorly understood organ. Due to its anatomic location and structural fragility, the omentum presents inherent challenges to mechanism-based in vivo studies. Thus, the availability of an ex vivo omental model would, in part, address some of these difficulties posed. Here we describe a technique for identifying, isolating and maintaining ex vivo cultures of omenta from immune-compromised and -competent mice. Ex vivo culture conditions were developed that maintain tissue viability, architecture, and function for up to 10 days. Further experiments demonstrate that the ex vivo culture conditions allow for the proliferation of ovarian cancer cells in vitro and support a similar pattern of microscopic lesions after either intraperitoneal injection of ovarian cancer cells or co-culture of ovarian cancer cells with the omentum. In agreement with previous studies from our laboratory, histologic evaluation of these specimens found that ovarian cancer cells, as well as other peritoneal cancer cells, preferentially accumulate in, and colonize, omental areas rich in immune cells. We now recognize that these are specific, functional structures referred to as milky spots. In sum, these are foundational studies of a readily accessible model, which is easily manipulated and can be immediately used to study the dynamic process of omental colonization. It is hoped that investigators will use the data herein as a starting point for refinements and modifications which will enable them to tailor the model to the specific needs of the experimental question(s) they wish to pursue.
KW - Immune aggregates
KW - Metastatic colonization
KW - Milky spots
KW - Omentum
KW - Ovarian cancer
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U2 - 10.1007/s10585-010-9317-0
DO - 10.1007/s10585-010-9317-0
M3 - Article
C2 - 20229256
AN - SCOPUS:77950861553
VL - 27
SP - 185
EP - 196
JO - Clinical and Experimental Metastasis
JF - Clinical and Experimental Metastasis
SN - 0262-0898
IS - 3
ER -