In vitro inhibition of rat liver cholest-5en-3β-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents

Darrell Abernethy, Charles Hignite, Daniel L. Azarnoff

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro conversion of 2-14C-mevalonate to cholest-5en-3β-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, β-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5α-cholest-8en-3β-ol and the other tentatively identified as 4,4 dimethyl-5α-cholest-8,24-dien-3β-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5α-lanosta-8,24-dien-3β-ol 14α demethylase.

Original languageEnglish (US)
Pages (from-to)297-307
Number of pages11
JournalSteroids
Volume27
Issue number3
DOIs
StatePublished - Mar 1976

Keywords

  • 24-Dihydrolanosterol
  • 5α-lanost-8en-33-ol
  • 5α-lanosta-8,24-dien-3β-ol
  • Cholest-5en-3β-ol
  • Cholesterol
  • Lanosterol

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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