In vitro immunological function of human corneal fibroblasts

E. Young, W. J. Stark

Research output: Contribution to journalArticlepeer-review

Abstract

Gamma-interferon-induced HLA-DR-like antigens on normal human fibroblasts were compared functionally to HLA-DR antigens on peripheral blood mononuclear leucocytes (PBML) from the same individual. Irradiated DR+ fibroblasts did not stimulate allogeneic PBML to divide in a mixed lymphocytic fibroblast culture (MLFC) as determined by 3H-thymidine incorporation, although lymphocytes autologous to the fibroblasts stimulated normally. Co-culture with interleukin 1 (IL-1), IL-2, 2-mercaptoethanol and indomethacin had no effect on MLFC. Pretreatment of stimulator fibroblasts with neuraminidase to modify sialoglycoproteins increased responses only modestly. Both DR+ and DR- fibroblasts inhibited a normal mixed lymphocyte culture (MLC) response in a dose-dependent fashion. These results suggest that the induction of HLA-DR-like molecules on fibroblasts is not sufficient to initiate host sensitization and that fibroblasts may inhibit normal lymphoproliferation.

Original languageEnglish (US)
Pages (from-to)1402-1406
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume29
Issue number9
StatePublished - 1988

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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