In vitro immunological function of human corneal fibroblasts

E. Young, W. J. Stark

Research output: Contribution to journalArticle

Abstract

Gamma-interferon-induced HLA-DR-like antigens on normal human fibroblasts were compared functionally to HLA-DR antigens on peripheral blood mononuclear leucocytes (PBML) from the same individual. Irradiated DR+ fibroblasts did not stimulate allogeneic PBML to divide in a mixed lymphocytic fibroblast culture (MLFC) as determined by 3H-thymidine incorporation, although lymphocytes autologous to the fibroblasts stimulated normally. Co-culture with interleukin 1 (IL-1), IL-2, 2-mercaptoethanol and indomethacin had no effect on MLFC. Pretreatment of stimulator fibroblasts with neuraminidase to modify sialoglycoproteins increased responses only modestly. Both DR+ and DR- fibroblasts inhibited a normal mixed lymphocyte culture (MLC) response in a dose-dependent fashion. These results suggest that the induction of HLA-DR-like molecules on fibroblasts is not sufficient to initiate host sensitization and that fibroblasts may inhibit normal lymphoproliferation.

Original languageEnglish (US)
Pages (from-to)1402-1406
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume29
Issue number9
StatePublished - 1988

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Fibroblasts
HLA-DR Antigens
Mononuclear Leukocytes
Lymphocytes
Sialoglycoproteins
In Vitro Techniques
Mercaptoethanol
Neuraminidase
Coculture Techniques
Interleukin-1
Indomethacin
Thymidine
Interferon-gamma
Interleukin-2

ASJC Scopus subject areas

  • Ophthalmology

Cite this

In vitro immunological function of human corneal fibroblasts. / Young, E.; Stark, W. J.

In: Investigative Ophthalmology and Visual Science, Vol. 29, No. 9, 1988, p. 1402-1406.

Research output: Contribution to journalArticle

Young, E. ; Stark, W. J. / In vitro immunological function of human corneal fibroblasts. In: Investigative Ophthalmology and Visual Science. 1988 ; Vol. 29, No. 9. pp. 1402-1406.
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