In vitro hypothermia enhances platelet GPIIb-IIIa activation and P- selectin expression

Nauder Faraday, Brian A. Rosenfeld

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


Background: A clinical bleeding diathesis is associated with hypothermia. Inhibition of platelet reactivity is the purported cause of this coagulopathy despite inconsistent evidence to support this hypothesis. To clarify the effect of temperature on intrinsic platelet function, platelet GPIIb-IIIa activation and P-selectin expression were assessed under normothermic and hypothermic conditions tn vitro. Methods: Blood was obtained by venipuncture from healthy volunteers. Platelet activation was assessed by aggregometry and by cytometric analysis of platelet binding of fibrinogen, PAC-1, and P-selectin antibodies. Measurements were made at normothermia (37°C), moderate hypothermia (33°C), and profound hypothermia (22°C) after stimulating samples with adenosine diphosphate (ADP), collagen, or thrombin receptor activating peptide. Results: Agonist-induced platelet aggregation and fibrinogen binding were significantly greater at 22°C and 33°C than at 37°C. Platelet fibrinogen binding values to 20 μM ADP were 23,400, 14,300, and 9,700 molecules/platelet at 22°C, 33°C, and 37°C, respectively. The aggregation responses of platelets that were cooled and rewarmed were indistinguishable from those of platelets maintained at 37°C throughout the study. Platelet binding of PAC-1 and P-selectin antibodies was greater under hypothermic conditions. Conclusions: Aggregation, fibrinogen binding, PAC-1 binding, and P-selectin antibody binding studies showed that platelet GPIIb- IIIa activation and α-granule release were enhanced at hypothermic temperatures. Thus hypothermia appears to increase the ability of platelets to respond to activating stimuli. The coagulopathy associated with hypothermia is not likely to be the result of an intrinsic defect in platelet function.

Original languageEnglish (US)
Pages (from-to)1579-1585
Number of pages7
Issue number6
StatePublished - 1998


  • Aggregation
  • Coagulopathy
  • GPIIb-IIIa
  • Hypothermia
  • P- selectin
  • Platelet
  • Temperature

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine


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