In vitro growth of murine T cells. V. The isolation and growth of lymphoid cells infiltrating syngeneic solid tumors

I. Yron, T. A. Wood, P. J. Spiess, S. A. Rosenberg

Research output: Contribution to journalArticle

Abstract

A method has been developed for isolating and growing lymphoid cells infiltrating murine solid tumors. When tumor cell suspensions are placed in T cell growth factor (TCGF) lymphoid cells and not tumor cells proliferate. By 7 to 9 days lymphoid cells free of tumor can be harvested and grown continuously in TCGF. The growth of these cells is dependent on the presence of TCGF but not on the presence of concanavalin-A. Tumor cell suspensions that have been freed of T lymphoid cells by treatment with anti-Thy-1.2 serum and complement or by fractionation on Ficoll gradients exhibit no cell outgrowth in TCGF. The lymphoid cells growing in TCGF exhibit significant cytotoxicity for syngeneic tumor cells and normal fibroblasts grown in culture but do not lyse normal lymphoid cells.

Original languageEnglish (US)
Pages (from-to)238-245
Number of pages8
JournalJournal of Immunology
Volume125
Issue number1
StatePublished - 1980
Externally publishedYes

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Interleukin-2
Lymphocytes
T-Lymphocytes
Growth
Neoplasms
Suspensions
Ficoll
Concanavalin A
In Vitro Techniques
Fibroblasts
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

In vitro growth of murine T cells. V. The isolation and growth of lymphoid cells infiltrating syngeneic solid tumors. / Yron, I.; Wood, T. A.; Spiess, P. J.; Rosenberg, S. A.

In: Journal of Immunology, Vol. 125, No. 1, 1980, p. 238-245.

Research output: Contribution to journalArticle

Yron, I. ; Wood, T. A. ; Spiess, P. J. ; Rosenberg, S. A. / In vitro growth of murine T cells. V. The isolation and growth of lymphoid cells infiltrating syngeneic solid tumors. In: Journal of Immunology. 1980 ; Vol. 125, No. 1. pp. 238-245.
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