In vitro growth of murine T cells: IV. Use of T-cell growth factor to clone lymphoid cells

Steven A. Rosenberg, Paul J. Spiess, Susan Schwarz

Research output: Contribution to journalArticle

Abstract

Soft agar and limiting dilution techniques have been used in conjunction with T-cell growth factor to develop methods for the cloning of continuous T-lymphoid populations from single lymphoid cells. Clones have been produced from both fresh murine splenocytes as well as from in vitro sensitized cells. These T-cell clones have continued to express specific cytotoxic functions for over 4 months and should be a valuable source of monoclonal lymphoid cells for immunologic studies.

Original languageEnglish (US)
Pages (from-to)293-306
Number of pages14
JournalCellular Immunology
Volume54
Issue number2
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'In vitro growth of murine T cells: IV. Use of T-cell growth factor to clone lymphoid cells'. Together they form a unique fingerprint.

  • Cite this