In vitro growth of murine T cells. IV. Use of T-cell growth factor to clone lymphoid cells

S. A. Rosenberg, P. J. Spiess, S. Schwarz

Research output: Contribution to journalArticle

Abstract

Soft agar and limiting dilution techniques have been used in conjunction with T-cell growth factor to develop methods for the cloning of continuous T-lymphoid populations from single lymphoid cells. Clones have been produced from both fresh murine splenocytes as well as from in vitro sensitized cells. These T-cell clones have continued to express specific cytotoxic functions for over 4 months and should be a valuable source of monoclonal lymphoid cells for immunologic studies.

Original languageEnglish (US)
Pages (from-to)293-306
Number of pages14
JournalCellular Immunology
Volume54
Issue number2
StatePublished - 1980
Externally publishedYes

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Interleukin-2
Clone Cells
Lymphocytes
T-Lymphocytes
Indicator Dilution Techniques
Growth
Agar
Organism Cloning
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Rosenberg, S. A., Spiess, P. J., & Schwarz, S. (1980). In vitro growth of murine T cells. IV. Use of T-cell growth factor to clone lymphoid cells. Cellular Immunology, 54(2), 293-306.

In vitro growth of murine T cells. IV. Use of T-cell growth factor to clone lymphoid cells. / Rosenberg, S. A.; Spiess, P. J.; Schwarz, S.

In: Cellular Immunology, Vol. 54, No. 2, 1980, p. 293-306.

Research output: Contribution to journalArticle

Rosenberg, SA, Spiess, PJ & Schwarz, S 1980, 'In vitro growth of murine T cells. IV. Use of T-cell growth factor to clone lymphoid cells', Cellular Immunology, vol. 54, no. 2, pp. 293-306.
Rosenberg, S. A. ; Spiess, P. J. ; Schwarz, S. / In vitro growth of murine T cells. IV. Use of T-cell growth factor to clone lymphoid cells. In: Cellular Immunology. 1980 ; Vol. 54, No. 2. pp. 293-306.
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