TY - JOUR
T1 - In vitro growth of murine T cells. III. Method for separation of T cell growth factor (TCGF) from concanavalin A and biological activity of the resulting TCGF
AU - Rosenberg, Steven A.
AU - Schwarz, Susan
AU - Spiess, Paul J.
AU - Brown, John M.
N1 - Funding Information:
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205, U.S.A.
PY - 1980
Y1 - 1980
N2 - T cell growth factor (TCGF) resulting from the incubation of murine splenocytes with concanavalin A (Con A) has been partially purified and separated from Con A. Sequential application of 50-70% saturated ammonium sulfate precipitation and G-100 gel filtration chromatography has resulted in a 300-fold purification of TCGF with a 60% yield. 99.99% of Con A has been removed from the TCGF by these steps. TCGF exists in two molecular weight forms of about 50,000 and 25,000 daltons. TCGF activity is degradable by trypsin digestion and is stable at 56°C for 30 min, but is inactivated by heating at 80°C. Lymphoid cells activated by either Con A or allogeneic in vitro sensitization will grow in TCGF free of Con A but fresh splenocytes will not grown in the absence of Con A, implying a need for prior activation before TCGF sustained growth of T cells can be achieved.
AB - T cell growth factor (TCGF) resulting from the incubation of murine splenocytes with concanavalin A (Con A) has been partially purified and separated from Con A. Sequential application of 50-70% saturated ammonium sulfate precipitation and G-100 gel filtration chromatography has resulted in a 300-fold purification of TCGF with a 60% yield. 99.99% of Con A has been removed from the TCGF by these steps. TCGF exists in two molecular weight forms of about 50,000 and 25,000 daltons. TCGF activity is degradable by trypsin digestion and is stable at 56°C for 30 min, but is inactivated by heating at 80°C. Lymphoid cells activated by either Con A or allogeneic in vitro sensitization will grow in TCGF free of Con A but fresh splenocytes will not grown in the absence of Con A, implying a need for prior activation before TCGF sustained growth of T cells can be achieved.
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U2 - 10.1016/0022-1759(80)90004-6
DO - 10.1016/0022-1759(80)90004-6
M3 - Article
C2 - 6966304
AN - SCOPUS:0018872530
SN - 0022-1759
VL - 33
SP - 337
EP - 350
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 4
ER -