In vitro growth of murine T cells. I. Production of factors necessary for T cell growth

S. A. Rosenberg, P. J. Spiess, S. Schwarz

Research output: Contribution to journalArticle

Abstract

Supernatants of murine lymphoid cultures stimulated with Concanavalin A contain factor(s) capable of sustaining continuous growth of murine lymphoid cells in vitro. Methods for the optimal generation of these growth factors (GM) have been defined, including number of cells required, optimal concentration of Concanavalin A, incubation time, and strain differences in GM production. With GM, cells have been grown for over 15 10-fold generations over a 2-month period. The growing cells express the θ surface marker and do not express surface Ia antigens.

Original languageEnglish (US)
Pages (from-to)1946-1950
Number of pages5
JournalJournal of Immunology
Volume121
Issue number5
StatePublished - Dec 1 1978

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Rosenberg, S. A., Spiess, P. J., & Schwarz, S. (1978). In vitro growth of murine T cells. I. Production of factors necessary for T cell growth. Journal of Immunology, 121(5), 1946-1950.