In vitro growth of cytotoxic human lymphocytes. II. Use of T cell growth factor (TCGF) to clone human T cells

M. T. Lotze, J. L. Strausser, S. A. Rosenberg

Research output: Contribution to journalArticle

Abstract

Soft agar and limiting dilution techniques utilizing human T cell growth factor (TCGF) have been developed to clone human lymphoid cells with high cloning efficiency. TCGF has been used for the continued growth and expansion of lymphoid populations derived from single T cells, and the cytotoxic specificity of individual clones has been repeately examined. An analysis of clones derived from human lymphocytes sensitized to allogeneic determinants in vitro reveals significant polymorphism in lytic recognition when tested against HLA typed targets. This pattern of reactivity remained constant over the 2 months of continuous culture. These methods should be capable of dissecting and analyzing the fine specificity of the T cell response.

Original languageEnglish (US)
Pages (from-to)2972-2978
Number of pages7
JournalJournal of Immunology
Volume124
Issue number6
StatePublished - Jan 1 1980

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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