In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens

J. L. Strausser, S. A. Rosenberg

Research output: Contribution to journalArticle


Human lymphocytes sensitized to allogeneic determinants in vitro were continuously cultured on medium prepared from phytohemagglutinin-(PHA-P) stimulated human mixed lymphocyte cultures. With such human-conditioned medium (HCM), human peripheral lymphoid cells could be grown in culture for over 90 days with a doubling time of about 54 hr. Cytotoxic lymphocytes could be grown in culture for this time with no loss of cytotoxicity or change in cytotoxic specificity.

Original languageEnglish (US)
Pages (from-to)1491-1495
Number of pages5
JournalJournal of Immunology
Issue number4
Publication statusPublished - 1978
Externally publishedYes


ASJC Scopus subject areas

  • Immunology

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