In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens

J. L. Strausser, S. A. Rosenberg

Research output: Contribution to journalArticle

Abstract

Human lymphocytes sensitized to allogeneic determinants in vitro were continuously cultured on medium prepared from phytohemagglutinin-(PHA-P) stimulated human mixed lymphocyte cultures. With such human-conditioned medium (HCM), human peripheral lymphoid cells could be grown in culture for over 90 days with a doubling time of about 54 hr. Cytotoxic lymphocytes could be grown in culture for this time with no loss of cytotoxicity or change in cytotoxic specificity.

Original languageEnglish (US)
Pages (from-to)1491-1495
Number of pages5
JournalJournal of Immunology
Volume121
Issue number4
StatePublished - 1978
Externally publishedYes

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Isoantigens
Lymphocytes
Growth
Conditioned Culture Medium
In Vitro Techniques
phytohemagglutinin-P

ASJC Scopus subject areas

  • Immunology

Cite this

In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens. / Strausser, J. L.; Rosenberg, S. A.

In: Journal of Immunology, Vol. 121, No. 4, 1978, p. 1491-1495.

Research output: Contribution to journalArticle

Strausser, J. L. ; Rosenberg, S. A. / In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens. In: Journal of Immunology. 1978 ; Vol. 121, No. 4. pp. 1491-1495.
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