Abstract
Human lymphocytes sensitized to allogeneic determinants in vitro were continuously cultured on medium prepared from phytohemagglutinin-(PHA-P) stimulated human mixed lymphocyte cultures. With such human-conditioned medium (HCM), human peripheral lymphoid cells could be grown in culture for over 90 days with a doubling time of about 54 hr. Cytotoxic lymphocytes could be grown in culture for this time with no loss of cytotoxicity or change in cytotoxic specificity.
Original language | English (US) |
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Pages (from-to) | 1491-1495 |
Number of pages | 5 |
Journal | Journal of Immunology |
Volume | 121 |
Issue number | 4 |
State | Published - Dec 1 1978 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology