In vitro growth and differentiation of mammalian sensory hair cell progenitors: A requirement for EGF and periotic mesenchyme

Angelika Doetzlhofer, Patricia M. White, Jane E. Johnson, Neil Segil, Andrew K. Groves

Research output: Contribution to journalArticle

Abstract

The sensory hair cells and supporting cells of the organ of Corti are generated by a precise program of coordinated cell division and differentiation. Since no regeneration occurs in the mature organ of Corti, loss of hair cells leads to deafness. To investigate the molecular basis of hair cell differentiation and their lack of regeneration, we have established a dissociated cell culture system in which sensory hair cells and supporting cells can be generated from mitotic precursors. By incorporating a Math1-GFP transgene expressed exclusively in hair cells, we have used this system to characterize the conditions required for the growth and differentiation of hair cells in culture. These conditions include a requirement for epidermal growth factor, as well as the presence of periotic mesenchymal cells. Lastly, we show that early postnatal cochlear tissue also contains cells that can divide and generate new sensory hair cells in vitro.

Original languageEnglish (US)
Pages (from-to)432-447
Number of pages16
JournalDevelopmental biology
Volume272
Issue number2
DOIs
StatePublished - Aug 15 2004

Keywords

  • Corti
  • Hair cell
  • Mesenchyme

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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