In vitro genetic analysis of the Tetrahymena self-splicing intron

Rachel Green, Andrew D. Ellington, Jack W. Szostak

Research output: Contribution to journalArticle

Abstract

THE availability of methods for the amplification of nucleic acid sequences1,2 allows the genetic analysis in vitro of the structural and functional properties of many nucleic acids. We have now developed an in vitro selection and amplification system, and used it to analyse the self-splicing Tetrahymena ribozyme. A much wider range of selective conditions can be used in vitro than is possible with standard in vivo methods, and many more variants can be handled in vitro than in vivo. This method can be used to isolate the wild-type ribozyme, and structural variants that are as active as the wild type, from a pool of over 250,000 variants in only three cycles of selection and amplification.

Original languageEnglish (US)
Pages (from-to)406-408
Number of pages3
JournalNature
Volume347
Issue number6291
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this