In vitro evolution of new ribozymes with polynucleotide kinase activity

Jon R. Lorsch, Jack W. Szostak

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA molecules consisting of an ATP-binding domain flanked by regions of random sequence. Different classes of kinases catalyse the transfer of the γ-thiophosphate of ATP-γS to the 5′-hydroxyl or to internal 2′-hydroxyls. An engineered version of one class is able to catalyse the transfer of thiophosphate from ATP-γS to the 5′-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalNature
Volume371
Issue number6492
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • General

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