In vitro electrophysiological mapping of stem cells

Seth Weinberg, Elizabeth A. Lipke, Leslie Tung

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The use of stem cells for cardiac regeneration is a revolutionary, emerging research area. For proper function as replacement tissue, stem cell-derived cardiomyocytes (SC-CMs) must electrically couple with the host cardiac tissue. Electrophysiological mapping techniques, including microelectrode array (MEA) and optical mapping, have been developed to study cardiomyocytes and cardiac cell monolayers, and these can be applied to study stem cells and SC-CMs. MEA recordings take extracellular measurements at numerous points across a small area of cell cultures and are used to assess electrical propagation during cell culture. Optical mapping uses fluorescent dyes to monitor electrophysiological changes in cells, most commonly transmembrane potential and intracellular calcium, and can be easily scaled to areas of different sizes. The materials and methods for MEA and optical mapping are presented here, together with detailed notes on their use, design, and fabrication. We also provide examples of voltage and calcium maps of mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs), obtained in our laboratory using optical mapping techniques.

Original languageEnglish (US)
Title of host publicationStem Cells for Myocardial Regeneration
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages215-237
Number of pages23
ISBN (Print)9781607617044
DOIs
StatePublished - Jan 1 2010

Publication series

NameMethods in Molecular Biology
Volume660
ISSN (Print)1064-3745

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Keywords

  • Cardiomyocyte
  • Electrophysiology
  • Embryonic stem cell
  • Microelectrode array
  • Optical mapping

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Weinberg, S., Lipke, E. A., & Tung, L. (2010). In vitro electrophysiological mapping of stem cells. In Stem Cells for Myocardial Regeneration: Methods and Protocols (pp. 215-237). (Methods in Molecular Biology; Vol. 660). Humana Press Inc.. https://doi.org/10.1007/978-1-60761-705-1_14