In vitro effects of mitomycin-C on human keratocytes

H. M. Sadeghi, B. Seitz, S. Hayashi, L. LaBree, P. J. McDonnell

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose. To quantify the in vitro antiproliferative and cytotoxic effects of mitomycin-C on human keratocytes for its potential to modulate corneal stromal wound healing. Methods. Cultured human keratocytes were exposed to various concentrations of mitomycin-C for periods of 5 minutes and 1 hour. Keratocyte proliferation and viability were assessed by phase-contrast microscopy, 3H-thymidine uptake, and electronic cell counting. Results. Cyctotoxic changes and inhibition of keratocyte proliferation exhibited after exposure to mitomycin-C were both dose- and time-dependent. The lowest concentrations to significantly (>50%) inhibited keratocyte proliferation after 5-minute and 1-hour exposures were 0.05 mg/ml (p=0.005) and 0.005 mg/ml (p<0.001), respectively. With a concentration of 0.5mg/ml of mitomycin-C, 80.5% of the cells remained viable as compared to control after 5 minutes of incubation (p<.001); 45.7% of the cells remained viable after 1 hour of incubation (p<0.001). The median inhibitory dose (ID50) and median lethal dose (LD50) of mitomycin-C after 1 hour of exposure differed by a magnitude of 58 (0.0048 vs. 0.28 mg/ml). Conclusions. Mitomycin-C has antiproliferative effects at concentrations below those cytotoxic to human keratocytes. If used after photorefractive keratectomy, the drug should be administered at an antiproliferative rather than cytotoxic concentration.

Original languageEnglish (US)
Pages (from-to)S188
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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