Abstract
BACKGROUND: The purpose of the present study is to quantify the in vitro antiproliferative and cytotoxic effects of mitomycin-C on human keratocytes for their potential to modulate corneal stromal wound healing. METHODS: Cultured human keratocytes were exposed to various concentrations of mitomycin-C for periods of 5 minutes and 1 hour. Keratocyte proliferation and viability were assessed by phase-contrast microscopy, 3H-thymidine uptake, and electronic cell counting. RESULTS: Cytotoxic changes and inhibition of keratocyte proliferation exhibited after exposure to mitomycin-C were both dose- and time-dependent. The lowest concentrations to significantly (>50%) inhibit keratocyte proliferation after 5-minute exposures were 0.05 mg/ml (P<.005) and after 1-hour exposures were 0.005 mg/ml (P<.001). At 5 minutes, ID50 was 0.038 mg/ml and LD50 was much higher than the greatest concentration tested (0.5 mg/ml). Mitomycin-C's median inhibitory dose (ID50) and median lethal dose (LD50) after 1 hour of exposure differed by a magnitude of 50 (0.0048 vs. 0.28 mg/ml). CONCLUSIONS: Mitomycin-C has antiproliferative effects at concentrations below those cytotoxic to human keratocytes. If used after photorefractive keratectomy, the drug should be administered at antiproliferative rather than cytotoxic concentrations.
Original language | English (US) |
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Pages (from-to) | 534-540 |
Number of pages | 7 |
Journal | Journal of Refractive Surgery |
Volume | 14 |
Issue number | 5 |
State | Published - Sep 1998 |
Externally published | Yes |
ASJC Scopus subject areas
- Surgery
- Ophthalmology