In vitro effects of mitomycin-C on human keratocytes

H. Mehrdad Sadeghi, Berthold Seitz, Seiji Hayashi, Laurie LaBree, Peter J McDonnell

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The purpose of the present study is to quantify the in vitro antiproliferative and cytotoxic effects of mitomycin-C on human keratocytes for their potential to modulate corneal stromal wound healing. METHODS: Cultured human keratocytes were exposed to various concentrations of mitomycin-C for periods of 5 minutes and 1 hour. Keratocyte proliferation and viability were assessed by phase-contrast microscopy, 3H-thymidine uptake, and electronic cell counting. RESULTS: Cytotoxic changes and inhibition of keratocyte proliferation exhibited after exposure to mitomycin-C were both dose- and time-dependent. The lowest concentrations to significantly (>50%) inhibit keratocyte proliferation after 5-minute exposures were 0.05 mg/ml (P50 was 0.038 mg/ml and LD50 was much higher than the greatest concentration tested (0.5 mg/ml). Mitomycin-C's median inhibitory dose (ID50) and median lethal dose (LD50) after 1 hour of exposure differed by a magnitude of 50 (0.0048 vs. 0.28 mg/ml). CONCLUSIONS: Mitomycin-C has antiproliferative effects at concentrations below those cytotoxic to human keratocytes. If used after photorefractive keratectomy, the drug should be administered at antiproliferative rather than cytotoxic concentrations.

Original languageEnglish (US)
Pages (from-to)534-540
Number of pages7
JournalJournal of Refractive Surgery
Volume14
Issue number5
StatePublished - Sep 1998
Externally publishedYes

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Mitomycin
Lethal Dose 50
Photorefractive Keratectomy
Phase-Contrast Microscopy
Wound Healing
Thymidine
In Vitro Techniques
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Sadeghi, H. M., Seitz, B., Hayashi, S., LaBree, L., & McDonnell, P. J. (1998). In vitro effects of mitomycin-C on human keratocytes. Journal of Refractive Surgery, 14(5), 534-540.

In vitro effects of mitomycin-C on human keratocytes. / Sadeghi, H. Mehrdad; Seitz, Berthold; Hayashi, Seiji; LaBree, Laurie; McDonnell, Peter J.

In: Journal of Refractive Surgery, Vol. 14, No. 5, 09.1998, p. 534-540.

Research output: Contribution to journalArticle

Sadeghi, HM, Seitz, B, Hayashi, S, LaBree, L & McDonnell, PJ 1998, 'In vitro effects of mitomycin-C on human keratocytes', Journal of Refractive Surgery, vol. 14, no. 5, pp. 534-540.
Sadeghi HM, Seitz B, Hayashi S, LaBree L, McDonnell PJ. In vitro effects of mitomycin-C on human keratocytes. Journal of Refractive Surgery. 1998 Sep;14(5):534-540.
Sadeghi, H. Mehrdad ; Seitz, Berthold ; Hayashi, Seiji ; LaBree, Laurie ; McDonnell, Peter J. / In vitro effects of mitomycin-C on human keratocytes. In: Journal of Refractive Surgery. 1998 ; Vol. 14, No. 5. pp. 534-540.
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