In vitro effects of mitomycin-C on human keratocytes

H. Mehrdad Sadeghi, Berthold Seitz, Seiji Hayashi, Laurie LaBree, Peter J. McDonnell

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: The purpose of the present study is to quantify the in vitro antiproliferative and cytotoxic effects of mitomycin-C on human keratocytes for their potential to modulate corneal stromal wound healing. METHODS: Cultured human keratocytes were exposed to various concentrations of mitomycin-C for periods of 5 minutes and 1 hour. Keratocyte proliferation and viability were assessed by phase-contrast microscopy, 3H-thymidine uptake, and electronic cell counting. RESULTS: Cytotoxic changes and inhibition of keratocyte proliferation exhibited after exposure to mitomycin-C were both dose- and time-dependent. The lowest concentrations to significantly (>50%) inhibit keratocyte proliferation after 5-minute exposures were 0.05 mg/ml (P<.005) and after 1-hour exposures were 0.005 mg/ml (P<.001). At 5 minutes, ID50 was 0.038 mg/ml and LD50 was much higher than the greatest concentration tested (0.5 mg/ml). Mitomycin-C's median inhibitory dose (ID50) and median lethal dose (LD50) after 1 hour of exposure differed by a magnitude of 50 (0.0048 vs. 0.28 mg/ml). CONCLUSIONS: Mitomycin-C has antiproliferative effects at concentrations below those cytotoxic to human keratocytes. If used after photorefractive keratectomy, the drug should be administered at antiproliferative rather than cytotoxic concentrations.

Original languageEnglish (US)
Pages (from-to)534-540
Number of pages7
JournalJournal of Refractive Surgery
Volume14
Issue number5
StatePublished - Sep 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Ophthalmology

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