The proper functioning of the many types of ocular epithelium, most notably the retinal pigment epithelium (RPE), is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated tissue culture dishes and Epithelial Growth Factor. These techniques increased culture success using pairs of donor eyes from 25% to 87%. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.
ASJC Scopus subject areas
- Sensory Systems