In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel

Christopher G. Williams, Tae Kyun Kim, Anya Taboas, Athar Malik, Paul Manson, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSCS) from skeletally mature goats were encapsulated in a photopolymerizing poly(ethylene glycol)-based hydrogel and cultured with or without transforming growth factor β1 (TGF) to study the potential for chondrogenesis in a hydrogel scaffold system amenable to minimally invasive implantation. Chondrogenic differentiation was evaluated by histological, biochemical, and RNA analyses for the expression of cartilage extracellular matrix components. The two control groups studied were MSCs cultured in monolayer and MSCs encapsulated in the hydrogel and cultured for 6 weeks in chondrogenic medium without TGF-β1 (6wk-TGF). The three experimental time points for encapsulated cells studied were 0 days (0d), 3 weeks, and 6 weeks in chondrogenic medium with TGF-β1 at 10 ng/ml (3wk+TGF and 6wk+TGF). MSCs proliferated in the hydrogels with TGF-β1. Glycosaminoglycan (GAG) and total collagen content of the hydrogels increased to 3.5% dry weight and 5.0% dry weight, respectively, in 6wk+TGF constructs. Immunobistochemistry revealed the presence of aggrecan, link protein, and type II collagen. Upregulation of aggrecan and type II collagen gene expression compared with monolayer MSCs was demonstrated. Type I collagen gene expression decreased from 3 to 6 weeks in the presence of TGF-β1. 6wk-TGF hydrogels produced no GAG and only moderate amounts of collagen. However, immunohistochemistry and RT-PCR demonstrated a small amount of spontaneous differentiation in this control group. This study demonstrates the ability to encapsulate MSCs to form cartilage-like tissue in vitro in a photopolymerizing hydrogel. This system may be useful for minimally invasive implantation, MSC differentiation, and engineering of composite tissue structures with multiple cellular phenotypes.

Original languageEnglish (US)
Pages (from-to)679-688
Number of pages10
JournalTissue Engineering
Volume9
Issue number4
DOIs
StatePublished - Aug 2003

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Chondrogenesis
Hydrogel
Transforming Growth Factors
Stem cells
Mesenchymal Stromal Cells
Hydrogels
Bone
Bone Marrow
Collagen
Aggrecans
Collagen Type II
Cartilage
Glycosaminoglycans
Gene expression
Monolayers
In Vitro Techniques
Intercellular Signaling Peptides and Proteins
Tissue
Gene Expression
Weights and Measures

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. / Williams, Christopher G.; Kim, Tae Kyun; Taboas, Anya; Malik, Athar; Manson, Paul; Elisseeff, Jennifer Hartt.

In: Tissue Engineering, Vol. 9, No. 4, 08.2003, p. 679-688.

Research output: Contribution to journalArticle

Williams, Christopher G. ; Kim, Tae Kyun ; Taboas, Anya ; Malik, Athar ; Manson, Paul ; Elisseeff, Jennifer Hartt. / In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. In: Tissue Engineering. 2003 ; Vol. 9, No. 4. pp. 679-688.
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