In vitro chemosensitivity testing of hematological cancers

Immunohistochemical detection of ornithine decarboxylase

Yongchun Wang, Yaccov J. Ashkenazl, Uriel Bachrach

Research output: Contribution to journalArticle

Abstract

A new method for in vitro chemosensitivity testing of human lymphoma and leukemia patients has been developed. The method is based on the use of ornithine decarboxylase (ODC), a universal marker of proliferation, which is expressed early during the cell cycle and has a short half-life. This marker was detected by a quantitative immunohistochemical analysis, using an ODC antibody and a FITC-linked second antibody, The in vitro chemosensitivity of lymphocytes from four normal individuals was tested by the immunohistochemical method. Lymphocytes from 25 cancer patients were also examined. In drug-sensitive cells, the intensity of the marker declined in the presence of the drug, whereas resistance to the drug was demonstrated by the presence of the marker. A good correlation was found between the predicted chemosensitivity and the outcome of the therapy. It has been suggested that this approach could be used for in vitro chemosensitivity testing of hematological cancers and most likely also for other malignancies.

Original languageEnglish (US)
Pages (from-to)797-805
Number of pages9
JournalAnti-Cancer Drugs
Volume10
Issue number9
StatePublished - 1999
Externally publishedYes

Fingerprint

Ornithine Decarboxylase
Lymphocytes
Neoplasms
Antibodies
Fluorescein-5-isothiocyanate
Drug Resistance
Pharmaceutical Preparations
Half-Life
Lymphoma
Cell Cycle
Leukemia
In Vitro Techniques
Therapeutics

Keywords

  • Anti-cancer drugs
  • Chemosensitivity
  • Leukemia
  • Lymphoma
  • Ornithine decarboxylase
  • Polyamines

ASJC Scopus subject areas

  • Pharmacology
  • Cancer Research
  • Oncology

Cite this

In vitro chemosensitivity testing of hematological cancers : Immunohistochemical detection of ornithine decarboxylase. / Wang, Yongchun; Ashkenazl, Yaccov J.; Bachrach, Uriel.

In: Anti-Cancer Drugs, Vol. 10, No. 9, 1999, p. 797-805.

Research output: Contribution to journalArticle

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