In vitro bactericidal efficacy of equine polymorphonuclear leukocytes against Corynebacterium equi.

J. A. Yager, S. F. Foster, Mary Christine Zink, J. F. Prescott, J. H. Lumsden

Research output: Contribution to journalArticle

Abstract

Polymorphonuclear leukocytes from adult horses were separated from whole blood, using a 2-step Percoll gradient, and were tested for bactericidal function against Corynebacterium equi. Staphylococcus aureus, an organism against which equine neutrophils have proved efficacy, was a positive control. The percentage of uptake after a 15-minute preincubation of the neutrophils and bacteria in the presence of normal horse serum was also calculated. The results indicated that equine neutrophils effectively phagocytosed and killed C equi and S aureus. The percentage of uptake for S aureus (95% +/- 3%) was greater than that for C equi (85% +/- 6%) (P less than 0.001), but the bactericidal efficacy was equivalent. More than 90% of the ingested or attached bacteria were destroyed during the 3-hour incubation period (mean percentage of C equi killed = 96 +/- 2%; mean percentage of S aureus killed = 91 +/- 8%). These results indicated that a failure of bacterial killing by neutrophils is unlikely to be important in the pathogenesis of C equi pneumonia in the horse.

Original languageEnglish (US)
Pages (from-to)438-440
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume47
Issue number2
StatePublished - Feb 1986
Externally publishedYes

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Rhodococcus equi
Equus
Horses
neutrophils
Neutrophils
horses
Bacteria
uptake mechanisms
bacteria
blood serum
Phagocytosis
pneumonia
Staphylococcus aureus
Pneumonia
pathogenesis
In Vitro Techniques
blood
organisms
Serum

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vitro bactericidal efficacy of equine polymorphonuclear leukocytes against Corynebacterium equi. / Yager, J. A.; Foster, S. F.; Zink, Mary Christine; Prescott, J. F.; Lumsden, J. H.

In: American Journal of Veterinary Research, Vol. 47, No. 2, 02.1986, p. 438-440.

Research output: Contribution to journalArticle

Yager, J. A. ; Foster, S. F. ; Zink, Mary Christine ; Prescott, J. F. ; Lumsden, J. H. / In vitro bactericidal efficacy of equine polymorphonuclear leukocytes against Corynebacterium equi. In: American Journal of Veterinary Research. 1986 ; Vol. 47, No. 2. pp. 438-440.
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