In vitro assays of breast cancer stem cells

Debangshu Samanta, Gregg L Semenza

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Aldehyde dehydrogenase and mammosphere assays enable the cost-effective quantification and characterization of cancer stem cells (CSCs) from cancer cell lines as well as cancer tissue. Here we describe the quantification of CSCs in breast cancer cell lines using aldehyde dehydrogenase and mammosphere assays under hypoxic (1% O2) and non-hypoxic (20% O2) culture conditions. Using this method, a significant enrichment of CSCs compared to bulk populations is observed when breast cancer cells are exposed to 1% O2 for 72 h.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages237-246
Number of pages10
Volume1742
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1742
ISSN (Print)1064-3745

Keywords

  • Breast cancer
  • Cancer stem cells (CSC)
  • FACS
  • Hypoxia
  • Hypoxia-inducible factors (HIFs)
  • Self-renewal
  • Tumor-initiating cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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