In vitro and in vivo studies of a rapid and selective breath test for tuberculosis based upon mycobacterial CO dehydrogenase

Mamoudou Maiga, Seong Won Choi, Viorel Atudorei, Mariama C. Maiga, Zachary D. Sharp, William Ramses Bishai, Graham S. Timmins

Research output: Contribution to journalArticle

Abstract

One of the major hurdles in treating tuberculosis (TB) is the time-consuming and difficult methodology for diagnosis. Stable-isotope breath tests hold great potential for rapidly diagnosing an infectious disease, monitoring therapy, and determining a bacterial phenotype in a rapid, point-of-care manner that does not require invasive sampling. Here we describe the preclinical development of a potentially highly selective TB diagnostic breath test based upon the organism's CO dehydrogenase activity. After development of the test in vitro, we were able to use the breath test to discriminate between infected and control rabbits, demonstrating that a diagnosis can potentially be made and also that a complex bacterial phenotype can be noninvasively and rapidly studied in the host.

Original languageEnglish (US)
Article numbere00990-14
JournalmBio
Volume5
Issue number2
DOIs
StatePublished - Apr 15 2014

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carbon monoxide dehydrogenase
Breath Tests
Tuberculosis
Point-of-Care Systems
Phenotype
Routine Diagnostic Tests
Isotopes
Communicable Diseases
Rabbits
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

In vitro and in vivo studies of a rapid and selective breath test for tuberculosis based upon mycobacterial CO dehydrogenase. / Maiga, Mamoudou; Choi, Seong Won; Atudorei, Viorel; Maiga, Mariama C.; Sharp, Zachary D.; Bishai, William Ramses; Timmins, Graham S.

In: mBio, Vol. 5, No. 2, e00990-14, 15.04.2014.

Research output: Contribution to journalArticle

Maiga, Mamoudou ; Choi, Seong Won ; Atudorei, Viorel ; Maiga, Mariama C. ; Sharp, Zachary D. ; Bishai, William Ramses ; Timmins, Graham S. / In vitro and in vivo studies of a rapid and selective breath test for tuberculosis based upon mycobacterial CO dehydrogenase. In: mBio. 2014 ; Vol. 5, No. 2.
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